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Spatial Transcriptomics of Pancreatic Tumors Reveal Distinct Compartmentalisation of Neutrophil Subsets [Spatial Transcriptomics]

ABSTRACT: Neutrophils are increasingly recognized as key players in the tumour immune response, and are associated with poor clinical outcomes. Multiple studies have characterised various neutrophil states within the tumor, with associated pro- and anti- tumoral functions. However, it is unknown if this heterogeneity of neutrophils is contributed by their surrounding tumor microenivironment. To answer this, we performed 10X Visium spatial transcriptomics on a murine model of pancreatic cancer. We discovered that terminally differentiated T3 neutrophils localise primarily in regions near hypoxic and glycolytic areas, while transitional immature T1 and mature T2 neutrophils localise towards the peripheral regions of the tumors. This results points to a spatially dependent role of T3 neutrophils, which may be critical to carry out pro-tumorigenic functions.

ORGANISM(S): Mus musculus

PROVIDER: GSE244534 | GEO | 2024/01/11

REPOSITORIES: GEO

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