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Epigenetic signature of human vitamin D3 and IL-10-conditioned regulatory DCs

ABSTRACT: To define the epigenetic signature of human DCregs generated in vitamin 14 D3 (vitD3) and IL-10 compared to immune stimulatory DCs (sDCs), we measured levels of DNA methylation by whole genome bisulfite sequencing (WGBS). Distinct DNA methylation patterns were acquired by DCregs compared to sDCs. These patterns were located mainly in transcriptional regulatory regions. Genes associated with these programs were enriched in STAT3-signaling and valine catabolism in DCregs; conversely, pro-inflammatory pathways e.g., pattern recognition receptor signaling, were enriched in sDCs. Further, DCreg differentially-methylated regions (DMRs) were enriched in binding motifs specific to the immunomodulatory transcription factor KLF11, while AP1 (Fos:Jun) transcription factor binding motifs were enriched in sDC DMRs. Using publicly-available data22 sets, we defined a common epigenetic signature shared between DCregs generated in vitD3 and IL-10, dexamethasone or vitD3 alone. These insights may help pave the way for design of epigenetic-based approaches to enhance production of DCregs as effective therapeutic agents.

ORGANISM(S): Homo sapiens

PROVIDER: GSE244577 | GEO | 2024/11/27

REPOSITORIES: GEO

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Epigenetic signature of human vitamin D3 and IL-10-conditioned regulatory DCs

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