Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comparison of lymphoblastoid cell gene expression with immunofluorescent chromosome banding

ABSTRACT: Metaphase chromosome staining was used to provide a high level overview of the pattern of histone modifications (H3K27ac, H3K27me3 and H3K4me3) at a single cell level in human lymphoblastois cells. These epigenomic banding patterns were related to various genomic features including gene and CpG island density as well as gene expression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE24459 | GEO | 2010/10/26

SECONDARY ACCESSION(S): PRJNA132703

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Comparison of lymphoblastoid cell gene expression with immunofluorescent chromosome banding

Project description:Metaphase chromosome staining was used to provide a high level overview of the pattern of histone modifications (H3K27ac, H3K27me3 and H3K4me3) at a single cell level in human lymphoblastois cells. These epigenomic banding patterns were related to various genomic features including gene and CpG island density as well as gene expression. A 3 array study using RNA extracted from 3 separate cultures of human lymphoblastoid cells as biological replicates

2010-10-26 | E-GEOD-24459 | biostudies-arrayexpress

Comparison of gene expression with immunofluorescent chromosome banding and ChIP-seq of histone modifications in cell-cycle sorted lymphoblastoid and HeLa cells [array]

Project description:Metaphase chromosome staining was used to provide a high level overview of the pattern of histone modifications (H3K27ac, H3K27me3 and H3K4me3) at a single cell level in human lymphoblastoidand HeLa cells. A higher resolution view was achieved using ChIP-seq in cell-cycle sorted cells. These epigenomic haplotypes were related to various genomic features including gene and CpG island density as well as gene expression.

2021-01-22 | GSE165237 | GEO

Right ventricular failure due to pulmonary banding

2013-06-11 | GSE47814 | GEO

Right ventricular failure due to pulmonary banding

Project description:Right ventricular failure was induced thourgh pulmonary banding in 11 pigs. Right ventricular failure was defined as a SRVP >50 mmHg during two hours. After right ventricular failure was induced, half the pigs were treatmed with a Glenn-shunt combined with pulmonary banding for one hour, and the other half served as control group with pulmonary banding only. The aim was to study the change in global gene expression during right ventricular failure due to pulmonary banding, and the effect of volume unloading during pulmonary banding. 11 pigs. Samples at the following time periods: 1) Baseline 2) Right ventricular failure 3) Treatment with modified Glenn-shunt/Control. After Right ventricular failure, pigs were divided into two groups a) Treatment with modified Glenn-shunt or b) Control group

2013-06-11 | E-GEOD-47814 | biostudies-arrayexpress

Altered expression of uc.323 during aortic banding-induced cardiac hypertrophy in mice

Project description:To investigate the changes in T-UCR (transcribed ultraconserved regions) transcription during aortic banding-induced cardiac hypertrophy, we performed lncRNA microarray analysis on the hearts of mice subjected to sham or aortic banding surgery.

2019-11-30 | GSE141165 | GEO

Transcriptional characterization of cardiac tissue of rat pups with pulmonary banding

Project description:Expression analysis of cardiac tissue of rat pups with and without pulmonary banding

2021-07-31 | GSE143745 | GEO

Metaphase-Chromosome-Organization

Project description:Metaphase-Chromosome-Organization

| PRJEB4743 | ENA

Transcription profiling of mouse treated by ascending aortic banding on wild type and transgenic mice overexpressing CHF1/Hey2 in the myocardium reveals CHF1/Hey2 promotes physiological hypertrophy in response to pressure overload

Project description:We have previously found that overexpression of CHF1/Hey2 in the myocardium prevents the development of phenylephrine-induced hypertrophy. To determine the role of CHF1/Hey2 in pressure overload hypertrophy, we performed ascending aortic banding on wild type and transgenic mice overexpressing CHF1/Hey2 in the myocardium. We found that both wild type and transgenic mice developed increased ventricular weight to body weight ratios one week after aortic banding. Wild type mice also developed decreased fractional shortening after one week when compared to preoperative echocardiograms and sham operated controls. Transgenic mice, in comparison, demonstrated preserved fractional shortening. Histological examination of explanted heart tissue demonstrated extensive fibrosis in wild type hearts, but minimal fibrosis in transgenic hearts. TUNEL staining demonstrated increased apoptosis in the wild type hearts but not in the transgenic hearts. Exposure of cultured neonatal myocytes from wild type and transgenic animals to hydrogen peroxide, a potent inducer of apoptosis, demonstrated increased apoptosis in the wild type cells. Gene Set Analysis of microarray data from wild type and transgenic hearts one week after banding revealed suppression and activation of multiple pathways involving apoptosis, cell signaling and biosynthesis. These findings demonstrate that CHF1/Hey2 promotes physiological over pathological hypertrophy in pressure overload through suppression of apoptosis and global regulation of multiple transcriptional pathways. Experiment Overall Design: Ascending aortic banding on four wild type and four transgenic mice were compared

2010-02-17 | E-GEOD-13031 | biostudies-arrayexpress

Comparison of gene expression with immunofluorescent chromosome banding and ChIP-seq of histone modifications in cell-cycle sorted lymphoblastoid and HeLa cells [array]

Project description:Comparison of gene expression with immunofluorescent chromosome banding and ChIP-seq of histone modifications in cell-cycle sorted lymphoblastoid and HeLa cells [array]

| PRJNA693801 | ENA

Metaphase-Chromosome-Organization

Project description:Mitotic chromosomes are among the most recognizable structures in the cell, yet for over a century their internal organization remains largely unsolved. We applied chromosome conformation capture methods, 5C and Hi-C, across the cell cycle and revealed two alternative three-dimensional folding states of the human genome. We show that the highly compartmentalized and cell-type-specific organization described previously for non-synchronous cells is restricted to interphase. In metaphase, we identify a homogenous folding state, which is locus-independent, common to all chromosomes, and consistent among cell types, suggesting a general principle of metaphase chromosome organization. Using polymer simulations, we find that metaphase Hi-C data is inconsistent with classic hierarchical models, and is instead best described by a linearly-organized longitudinally compressed array of consecutive chromatin loops.

2013-11-05 | E-MTAB-1948 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data