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Biodiversity and functional genomics of endogenous small RNAs induced upon biotic and abiotic stresses in plant (gemsup)

ABSTRACT: au07-12_geminiselsup - gemsup - Determine the level of expresison in transgenic plants expressing geminviral gene silencing suppressors. - Transgenic lines of Arabidopsis overexpressing three different geminiviral silencing suppressors: C2, C4 and V2 were grown in adition to a control and their total RNA was extracted in order to study their genetic expressions profiles. Keywords: gene knock in (transgenic)

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE24475 | GEO | 2010/10/12

SECONDARY ACCESSION(S): PRJNA132777

REPOSITORIES: GEO

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Project description:BackgroundSmall RNAs (19-24 nt) are key regulators of gene expression that guide both transcriptional and post-transcriptional silencing mechanisms in eukaryotes. Current studies have demonstrated that microRNAs (miRNAs) act in several plant pathways associated with tissue proliferation, differentiation, and development and in response to abiotic and biotic stresses. In order to identify new miRNAs in soybean and to verify those that are possibly water deficit and rust-stress regulated, eight libraries of small RNAs were constructed and submitted to Solexa sequencing.ResultsThe libraries were developed from drought-sensitive and tolerant seedlings and rust-susceptible and resistant soybeans with or without stressors. Sequencing the library and subsequent analyses detected 256 miRNAs. From this total, we identified 24 families of novel miRNAs that had not been reported before, six families of conserved miRNAs that exist in other plants species, and 22 families previously reported in soybean. We also observed the presence of several isomiRNAs during our analyses. To validate novel miRNAs, we performed RT-qPCR across the eight different libraries. Among the 11 miRNAs analyzed, all showed different expression profiles during biotic and abiotic stresses to soybean. The majority of miRNAs were up-regulated during water deficit stress in the sensitive plants. However, for the tolerant genotype, most of the miRNAs were down regulated. The pattern of miRNAs expression was also different for the distinct genotypes submitted to the pathogen stress. Most miRNAs were down regulated during the fungus infection in the susceptible genotype; however, in the resistant genotype, most miRNAs did not vary during rust attack. A prediction of the putative targets was carried out for conserved and novel miRNAs families.ConclusionsValidation of our results with quantitative RT-qPCR revealed that Solexa sequencing is a powerful tool for miRNA discovery. The identification of differentially expressed plant miRNAs provides molecular evidence for the possible involvement of miRNAs in the process of water deficit- and rust-stress responses.

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Biodiversity and functional genomics of endogenous small RNAs induced upon biotic and abiotic stresses in plant (gemsup)

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