Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Elucidating the mechanisms underlying H3K18la contributes to NSCLC progression

ABSTRACT: Histone lactylation has been served as a novel epigenetic modification that directly stimulates gene transcription from chromatin. In order to study the regulatory mechanisms of H3K18la in non-small cell lung cancer (NSCLC), we performed ChIP-Seq to identify its target genes in NCI-H1299 cell lines.

ORGANISM(S): Homo sapiens

PROVIDER: GSE245990 | GEO | 2024/08/06

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Elucidating the mechanisms underlying H3K18la contributes to NSCLC progression

Project description:Elucidating the mechanisms underlying H3K18la contributes to NSCLC progression

| PRJNA1030936 | ENA

The genome-wide targeting of H3K18la in H1299 cells

Project description:L-lactate was reported as a precursor that can label and stimulate histone lysine-N-L-lactylation (Kla), which represents a new epigenetic mark affecting gene expression directly via histone PTMs under conditions of high glycolysis, such as the Warburg effect. To investigate the genome-wide targeting of H3K18la, we performed ChIP-seq in H1299 cells using anti-H3K18la antibody. 50.6% of the H3K18la binding sites displayed enrichment close to -1kb promoter of genes. More importantly, our ChIP-seq data showed that H3K18la is enriched in many genes that related with replication processes, highlighted the importance of histone Kla involved in DNA replication.

2023-07-30 | GSE207814 | GEO

The genome-wide targeting of H3K18la in H1299 cells

Project description:The genome-wide targeting of H3K18la in H1299 cells

| PRJNA857271 | ENA

H3K18la marks active tissue-specific enhancers [RNA-Seq]

Project description:We profiled the genome-wide distribution of H3K18la in samples originating from 6 different in vitro and in vivo mouse samples, representing 3 tissues: embryonic stem cells, macrophages and skeletal muscle as well as in human skeletal muscle and compared them to the profiles of other well-established histone modifications as well as gene expression patterns. Globally, we found that H3K18la profiles resemble H3K27ac profiles better than any other investigated hPTM, including H3K4me3, but that they do not copy them. For all samples, H3K18la marked active CGI promoters of highly expressed genes which were remarkably shared across the different mouse tissues and which contained many housekeeping genes. Promoter H3K18la levels correlated positively to both H3K27ac and H3K4me3 levels as well as to gene expression levels. In addition, we found that H3K18la is enriched at tissue-type specific, active enhancers, which are particularly tissue-type-specific, especially when compared to the H3K18la-marked promoter regions. Accordingly, enhancer H3K18la levels correlate positively to the expression of their nearest genes. Additionally, genes closest to enhancers with high H3K18la levels predominantly consist of tissue-type specific marker genes. Overall, we showed that H3K18la is not only a marker for active promoters, but that it also marks active enhancers, and this both in embryonic tissues and differentiated tissues, and both in mouse and in human.

2022-09-14 | GSE195859 | GEO

H3K18la marks active tissue-specific enhancers [Cut & Tag mouse]

2022-09-14 | GSE195856 | GEO

H3K18la marks active tissue-specific enhancers [Cut & Tag human]

2022-09-14 | GSE195854 | GEO

Gene expression profiling by adenoviral CYB5R3 overexpression in non-small cell lung cancer (NSCLC) cell line, NCI-H1299

Project description:To investigate the tumor suppressor roleof CYB5R3 in lung cancer, we infected with adenoviral empty vector (EV) or CYB5R3 in NCI-H1299 cells.

2024-04-20 | GSE229344 | GEO

H3K18la marks active tissue-specific enhancers

Project description:H3K18la marks active tissue-specific enhancers

| PRJNA802354 | ENA

ZBTB11 and SET regulates genes in NCI-H1299

Project description:To determined ZBTB11 and SET regulates genes in NCI-H1299, we esteblished NCI-H1299 cell lines in which ZBTB11 and SET has been knocked down by si-RNA. We then conducted differential expressed genes analysis using data generated form RNA-seq of H1299 cell lines at the condition of two genes knocked down.

2023-12-21 | GSE206957 | GEO

Expression data from NCI-H1299 and NCI-H1299/CDDP cells

Project description:Expression data from NCI-H1299 and NCI-H1299/CDDP cells

| PRJNA1062721 | ENA