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Comparison of the response of Col-0 and ate1 ate2 N-degron mutant to flg22

ABSTRACT: Arabidopsis mutants of the N-degron pathway have been shown to be either susceptible or resistant to a wide range of pathogens. This includes mutants for the arginine-transferases (ate1-2 ate2-1) and for the downstream E3 ubiquitin ligase PRT6 (prt6). In addition, ate1-2 ate2-1 mutants have been shown to have dampened gene expression changes upon inoculation with Pseudomonas syringae AvrRpm1. Here, we tested whether ate1-2 ate2-1 mutants were impaired for pattern-triggered immunity (PTI) and determined gene expression changes of wild-type Col-0 and of ate1-2 ate2-1 mutant seedlings in the absence/pressence of flg22.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE246173 | GEO | 2024/07/24

REPOSITORIES: GEO

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Project description:In this research a high-throughput RNA sequencing based transcriptome analysis technique (RNA-Seq) was used to evaluate differentially expressed genes (DEGs) in the wild type Arabidopsis seedling in response to flg22, a well-known microbe-associated molecular patterns (MAMP), and AtPep1, a well-known peptide representing an endogenous damage-associated molecular patterns (DAMP). The results of our study revealed that 1895 (1634 up-regulated and 261 down-regulated) and 2271 (1706 up-regulated and 565 down-regulated) significant differentially expressed genes in response to flg22 and AtPep1 treatment, respectively. Among significant DEGs, we observed that a number of hitherto overlooked genes have been found to be induced upon treatment with either flg22 or with AtPep1, indicating their possible involvement in innate immunity. Here, we characterized two of them, namely PP2-B13 and ACLP1. PP2-B13 contains an F-box domain and shows similarity to carbohydrate binding proteins. ACLP1 is a protein of unknown function with highest similarity to actin cross linking proteins and includes a fascin domain. Using qPCR, we verified that the genes encoding PP2-B13, and ACLP1 were highly induced upon treatment of leaf disks with flg22. We obtained T-DNA insertion mutants and generated homozygous mutant lines. None of the mutants showed a phenotype in the absence of infection. pp2-b13 and aclp1 mutants showed an increased susceptibility to infection by the virulent pathogen Pseudomomas syringae pv tomato mutant hrcC-, as evidenced by an increased growth of the pathogen in planta. Further we present evidence that aclp1 was deficient in ethylene production upon flg22 treatment, while pp2-b13, was deficient in ROS production. In conclusion, the products of these genes contribute to plant immunity against bacterial pathogens, although there is currently no clue for their mechanism of action. The results from this research provide new information to a better understanding of the immune system in Arabidopsis.

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Comparison of the response of Col-0 and ate1 ate2 N-degron mutant to flg22

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