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Transcriptome investigation shed light into the role of volatile organic compounds in the growth inhibition of Xanthomonas species

ABSTRACT: X. albilineans is one of the most important phytobacteria species which affect sugarcane production. Volatile organic compounds (VOCs) produced by microorganisms may have a noteworthy role in the control of plant diseases. Thus, this study investigated VOC-producing soil bacteria with an antagonistic effect against X. albilineans and evaluated the molecular mechanisms of growth inhibition trigged by the volatile dimethyl disulfide (DMDS). The comparative transcriptomic data of X. albilineans treated with DMDS showed that several metabolic pathways are up-regulated, such as two-component system, flagellar assembly, chemotaxis and bacterial secretion system. Interesting, although the ethanol used as DMDS solvent did not inhibit X. albilineans growth, it triggers a similar gene up-regulation and somehow, the phytopathogen can deal with this harmful compound better than DMDS.

ORGANISM(S): Xanthomonas albilineans

PROVIDER: GSE246249 | GEO | 2025/01/31

REPOSITORIES: GEO

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			Action	DRS
		Other
	GSE246249_X_albilineans_TPM.tsv.gz	Tabular
	GSE246249_X_albilineans_edgeR_DE.tsv.gz	Tabular
	GSE246249_X_albilineans_raw_counts.tsv.gz	Tabular

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Project description:The oomycete P. oligandrum is a soil-inhabiting parasite and predator of both fungi and oomycetes and uses hydrolytic enzymes extensively to penetrate and hydrolyze its host or prey. Other mechanisms have been overlooked, and we investigated whether P. oligandrum-produced volatile organic compounds (VOCs) could also be contributing to antagonism. VOCs have diverse functions, including contributing to antagonism in ecological interactions and potential applications in biocontrol. The growth-inhibiting activity of P. oligandrum VOCs was tested on P. myriotylum – a host or prey of P. oligandrum – coupled with electron microscopy, biochemical assays and transcriptomic analysis. The total VOCs produced by P. oligandrum reduced P. myriotylum growth by 80% and zoospore levels by 60%. GC-MS identified twenty-three VOCs, and methyl heptenone, D-limonene, 2-undecanone and 1-octanal were potent inhibitors of P. myriotylum growth and led to increased production of reactive oxygen species at a concentration that did not inhibit P. oligandrum growth. Exposure to the total VOCs of P. oligandrum led to shrinkage of P. myriotylum hyphae, and lysis of the cellular membranes and organelles. Transcriptomic analysis of P. myriotylum exposed to the P. oligandrum VOCs at increasing levels of growth inhibition showed initially a strong upregulation of putative detoxification related genes that was not maintained later during exposure to the VOCs. The inhibition of P. myriotylum growth continued after the exposure to the VOCs was discontinued and led to reduced leaf lesion size during P. myriotylum infection of its plant host. The VOCs produced by P. oligandrum could be another factor alongside hydrolytic enzymes contributing to its ecological role as a microbial antagonist where the concentration of the VOCs may be inhibitory in particular ecological niches such as in soil. The VOCs analyzed here may also be contributing to the biocontrol of diseases using P. oligandrum commercial preparations.

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Transcriptome investigation shed light into the role of volatile organic compounds in the growth inhibition of Xanthomonas species

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