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The H2Bub1-deposition complex is required for human and mouse cardiogenesis

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE246413 | GEO | 2023/10/31

REPOSITORIES: GEO

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The H2Bub1-deposition complex is required for human and mouse cardiogenesis.

Project description:De novo variants affecting monoubiquitylation of histone H2B (H2Bub1) are enriched in human congenital heart disease. H2Bub1 is required in stem cell differentiation, cilia function, post-natal cardiomyocyte maturation and transcriptional elongation. However, how H2Bub1 affects cardiogenesis is unknown. We show that the H2Bub1-deposition complex (RNF20-RNF40-UBE2B) is required for mouse cardiogenesis and for differentiation of human iPSCs into cardiomyocytes. Mice with cardiac-specific Rnf20 deletion are embryonic lethal and have abnormal myocardium. We then analyzed H2Bub1 marks during differentiation of human iPSCs into cardiomyocytes. H2Bub1 is erased from most genes at the transition from cardiac mesoderm to cardiac progenitor cells but is preserved on a subset of long cardiac-specific genes. When H2Bub1 is reduced in iPSC-derived cardiomyocytes, long cardiac-specific genes have fewer full-length transcripts. This correlates with H2Bub1 accumulation near the center of these genes. H2Bub1 accumulation near the center of tissue-specific genes was also observed in embryonic fibroblasts and fetal osteoblasts. In summary, we show that normal H2Bub1 distribution is required for cardiogenesis and cardiomyocyte differentiation, and suggest that H2Bub1 regulates tissue-specific gene expression by increasing the amount of full-length transcripts.

| S-EPMC10730087 | biostudies-literature

The H2Bub1-deposition complex is required for human and mouse cardiogenesis (ChIP-Seq)

Project description:De novo variants affecting monoubiquitination of histone H2B (H2Bub1) are enriched in human congenital heart disease. H2Bub1 is required in stem cell differentiation, cilia function, post-natal cardiomyocyte maturation, and transcriptional elongation. However, how H2Bub1 affects cardiogenesis is unknown. We show that the H2Bub1-deposition complex (RNF20-RNF40-UBE2B) is required for mouse cardiogenesis and for differentiation of human iPSCs into cardiomyocytes. Mice with cardiac-specific Rnf20 deletion are embryonic lethal and have abnormal myocardium. We then analyzed H2Bub1 marks during differentiation of human iPSCs into cardiomyocytes. H2Bub1 is erased from most genes at transition from cardiac mesoderm to cardiac progenitor cells, but is preserved on a subset of long cardiac-specific genes. When H2Bub1 is reduced in iPSC-cardiomyocytes, long cardiac-specific genes have fewer full-length transcripts. This correlates with H2Bub1 accumulation near the center of these genes. H2Bub1 accumulation near the center of tissue-specific genes was also observed in embryonic fibroblasts and fetal osteoblasts. In summary, we show that normal H2Bub1 distribution is required for cardiogenesis and cardiomyocyte differentiation, and point to H2Bub1 regulating tissue-specific gene expression by increasing the amount of full-length transcripts.

2023-10-31 | GSE246410 | GEO

The H2Bub1-deposition complex is required for human and mouse cardiogenesis (RNA-Seq)

2023-10-31 | GSE246411 | GEO

Complex Interdependence Regulates Heterotypic Transcription Factor Distribution and Coordinates Cardiogenesis

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-02-11 | E-GEOD-72223 | biostudies-arrayexpress

The H2Bub1-deposition complex is required for human and mouse cardiogenesis

Project description:The H2Bub1-deposition complex is required for human and mouse cardiogenesis

| PRJNA1032891 | ENA

Complex Interdependence Regulates Heterotypic Transcription Factor Distribution and Coordinates Cardiogenesis

Project description:This SuperSeries is composed of the SubSeries listed below.

2016-02-11 | GSE72223 | GEO

The H2Bub1-deposition complex is required for human and mouse cardiogenesis (RNA-Seq)

Project description:The H2Bub1-deposition complex is required for human and mouse cardiogenesis (RNA-Seq)

| PRJNA1032897 | ENA

The H2Bub1-deposition complex is required for human and mouse cardiogenesis (ChIP-Seq)

Project description:The H2Bub1-deposition complex is required for human and mouse cardiogenesis (ChIP-Seq)

| PRJNA1032889 | ENA

Scl specifies hemogenic endothelium and inhibits cardiogenesis via primed enhancers

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-01-01 | E-GEOD-47085 | biostudies-arrayexpress

Histone H2Bub1 deubiquitylation is essential for mouse development, but does not regulate global RNA

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-02-16 | GSE153587 | GEO

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