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Structural Basis for Cytoplasmic Factors Required for Integrator and CPSF Function

ABSTRACT: The object of this study (or experiment) was to determine the impact of BRAT1 depletion on the transcriptome during differentiation into neuroprogenitor cells. Two control and two BRAT1 knockout H7-human ESC lines were differentiated for 14 days, total RNA isolated and polyA enriched Click-seq libraries generated. Broad transcriptional misregulation was observed between differentiated knockout and control lines. Specifically, the precocious upregulation of several neurogenesis-driving transcription factors indicates the mistiming of differentiation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE246833 | GEO | 2024/07/22

REPOSITORIES: GEO

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Nuclear-localized Asunder regulates cytoplasmic dynein localization via its role in the integrator complex.

Project description:We previously reported that Asunder (ASUN) is essential for recruitment of dynein motors to the nuclear envelope (NE) and nucleus-centrosome coupling at the onset of cell division in cultured human cells and Drosophila spermatocytes, although the mechanisms underlying this regulation remain unknown. We also identified ASUN as a functional component of Integrator (INT), a multisubunit complex required for 3'-end processing of small nuclear RNAs. We now provide evidence that ASUN acts in the nucleus in concert with other INT components to mediate recruitment of dynein to the NE. Knockdown of other individual INT subunits in HeLa cells recapitulates the loss of perinuclear dynein in ASUN-small interfering RNA cells. Forced localization of ASUN to the cytoplasm via mutation of its nuclear localization sequence blocks its capacity to restore perinuclear dynein in both cultured human cells lacking ASUN and Drosophila asun spermatocytes. In addition, the levels of several INT subunits are reduced at G2/M when dynein is recruited to the NE, suggesting that INT does not directly mediate this step. Taken together, our data support a model in which a nuclear INT complex promotes recruitment of cytoplasmic dynein to the NE, possibly via a mechanism involving RNA processing.

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