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Exosomal miR-302b rejuvenates aging mice by reversing cell cycle arrest [LO2 scRNA-Seq]

ABSTRACT: Cellular senescence is a hallmark of aging characterized by a stable exit from the cell cycle in response to cellular damage and stress. Senescent cells (SnCs) are closely associated with aging and aging-related disorders, making them a potential target for slowing aging. In this study, we developed a tool for dynamically monitoring the p21 signaling to determine the degree of cellular senescence, named p21-YFP LO2. The senescent cells were prepared by FCM sorting targeting p21-YFP LO2 treated with 50 nM Dox for 48 h based on the YFP signaling. The single-cell RNA sequencing (scRN-seq) analysis was performed to observe the effects of human embryonic stem cell-derived exosomes (hESC-Exos) on the transcriptional profiles of SnCs. We specifically demonstrated that hESC-Exos enable SnCs to reverse the cell cycle arrest and restore their proliferative capacity in vitro.

ORGANISM(S): Homo sapiens

PROVIDER: GSE247073 | GEO | 2024/11/01

REPOSITORIES: GEO

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Exosomal miR-302b rejuvenates aging mice by reversing cell cycle arrest [scRNA-Seq]

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2024-11-01 | GSE247074 | GEO

Programmed cell senescence during embryonic development

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Expression data of growing and senescent human fibroblasts (BJ cells) after p21 knockdown

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Transfer learning of an in vivo-derived senescence signature identifies conserved tissue-specific senescence across species and diverse pathologies [bulk RNA-seq]

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2013-11-14 | GSE49108 | GEO

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