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Transcriptome analysis reveals the mechanism of bovine lactoferrin against lipopolysaccharide-induced acute lung injury

ABSTRACT: Lactoferrin (LF) is an iron-binding multifunctional cationic glycoprotein secreted by exocrine glands and neutrophils in mammals. In this study, we created a mouse model of lipopolysaccharide (LPS)-induced acute lung injury and explored the anti-inflammatory effect and mechanism of bovine lactoferrin (bLF) in acute lung injury using the RNA sequencing (RNA-seq) technology and transcriptome analysis.

ORGANISM(S): Mus musculus

PROVIDER: GSE247266 | GEO | 2023/12/19

REPOSITORIES: GEO

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Gene expression profiling in HEK293 cells overexpressed lactoferrin gene that has the signal sequence (sLF) or not (ΔLF)

Project description:Human lactoferrin (LF) is a multifunctional protein involved in immunomodulation, cell growth, and differentiation. In addition to the secreted form (sLF), an alternatively spliced form (ΔLF) that lacks the signal sequence and downregulated in cancer was found. This study was carried out to identify and compare signaling networks provoked by the two LF isoforms. To do this, the two forms were overexpressed in HEK293 cells using the flp-in/tet-on system and genome-wide expression analysis of 18,367 genes was conducted.

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Transcriptome analysis reveals the mechanism of bovine lactoferrin against lipopolysaccharide-induced acute lung injury

Project description:Transcriptome analysis reveals the mechanism of bovine lactoferrin against lipopolysaccharide-induced acute lung injury

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Expression data from Caenorhabditis elegans fed with a Lactoferrin-based product

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