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PELOTA and HBS1 suppress co-translational messenger RNA decay in Arabidopsis

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE247330 | GEO | 2023/12/31

REPOSITORIES: GEO

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PELOTA and HBS1 suppress co-translational messenger RNA decay in Arabidopsis [GMUCT]

Project description:Various messenger RNA (mRNA) decay mechanisms play major roles in controlling mRNA quality and quantity in eukaryotic organisms under different conditions. While it is known that the recently discovered co-translational mRNA decay (CTRD), the mechanism that allows mRNAs to be degraded while still being actively translated, is prevalent in yeast, human, and various angiosperms, the regulation of this decay mechanism is less well studied. Moreover, it is still unclear whether this decay mechanism plays any roles in regulation of specific physiological processes in eukaryotes. Here, by re-analyzing the publicly available polysome profiling or ribosome footprinting and degradome sequencing datasets, we discovered that highly translated mRNAs tend to have lower co-translational decay levels. Based on this finding, we then identified Pelota and Hbs1, the translation-related ribosome rescue factors, as suppressors of co-translational mRNA decay in Arabidopsis. Furthermore, we found that Pelota and Hbs1 null mutants have lower germination rates compared to the wild type plants, implying that proper regulation of co-translational mRNA decay is essential for normal developmental processes. In total, our study provides further insights into the regulation of CTRD in Arabidopsis and demonstrates that this decay mechanism does play important roles in Arabidopsis physiological processes.

2023-12-31 | GSE247329 | GEO

PELOTA and HBS1 suppress co-translational messenger RNA decay in Arabidopsis [mRNA-Seq]

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PELOTA and HBS1 suppress co-translational messenger RNA decay in Arabidopsis

Project description:PELOTA and HBS1 suppress co-translational messenger RNA decay in Arabidopsis

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PELOTA and HBS1 suppress co-translational messenger RNA decay in Arabidopsis [GMUCT]

Project description:PELOTA and HBS1 suppress co-translational messenger RNA decay in Arabidopsis [GMUCT]

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PELOTA and HBS1 suppress co-translational messenger RNA decay in Arabidopsis [mRNA-Seq]

Project description:PELOTA and HBS1 suppress co-translational messenger RNA decay in Arabidopsis [mRNA-Seq]

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Heat-induced ribosome pausing triggers mRNA co-translational decay in Arabidopsis thaliana.

Project description:The reprogramming of gene expression in heat stress is a key determinant to organism survival. Gene expression is downregulated through translation initiation inhibition and release of free mRNPs that are rapidly degraded or stored. In mammals, heat also triggers 5'-ribosome pausing preferentially on transcripts coding for HSC/HSP70 chaperone targets, but the impact of such phenomenon on mRNA fate remains unknown. Here, we provide evidence that, in Arabidopsis thaliana, heat provokes 5'-ribosome pausing leading to the XRN4-mediated 5'-directed decay of translating mRNAs. We also show that hindering HSC/HSP70 activity at 20°C recapitulates heat effects by inducing ribosome pausing and co-translational mRNA turnover. Strikingly, co-translational decay targets encode proteins with high HSC/HSP70 binding scores and hydrophobic N-termini, two characteristics that were previously observed for transcripts most prone to pausing in animals. This work suggests for the first time that stress-induced variation of translation elongation rate is an evolutionarily conserved process leading to the polysomal degradation of thousands of 'non-aberrant' mRNAs.

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Project description:This SuperSeries is composed of the SubSeries listed below.

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Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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