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CBir1 T cells acquire an effector phenotype during inflammation (ATAC-seq)

ABSTRACT: CD4+ T cells specific for commensal bacterial antigens are expanded in inflammatory bowel disease (IBD) patients compared to healthy individuals. How and where commensal-specific CD4+ T cells get activated is yet to be fully understood. We used TCR-transgenic CD4+ T cells specific to a commensal bacterial antigen (CBir1 T cells) and the dextran sulfate sodium (DSS) model of IBD to characterize how and where the activation of commensal-specific CD4+ T cells occurs. We found that CBir1 T cells proliferate following intestinal damage and cognate antigen presentation is mediated by CD11c+ cells in the colon-draining mesenteric lymph nodes (cMLNs). Using assay for transposase-accessible chromatin sequencing (ATAC-seq) and flow cytometry, we showed that activated CBir1 T cells preferentially acquire an effector rather than regulatory phenotype, which is plastic over time.

ORGANISM(S): Mus musculus

PROVIDER: GSE247375 | GEO | 2023/11/15

REPOSITORIES: GEO

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