Methylation profiling

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Effect of gut microbiome transplant from neonates of particle-exposed mothers on the epigenome of dendritic cells


ABSTRACT: In our model the newborns of asthmatic mother mice or of mothers exposed to air pollutant particles are born with a predisposition to asthma. Gut microbiome of these pups is altered, and the transplant of the pups’ microbiome (GMT) has conferred the asthma predisposition to naïve recipients. We hypothesized that bacteria alter metabolomic profile in the gut, which polarizes the dendritic cells (DC) in the recipient by affecting epigenetic regulation in these key decision-maker cells. Here we examined DNA methylation profiles in the recipient host’s DCs to test the prediction that GMT confers alterations in DNA methylation (not seen with sterilized GMT).

ORGANISM(S): Mus musculus

PROVIDER: GSE247470 | GEO | 2024/04/01

REPOSITORIES: GEO

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