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Expression data from stem samples taken from the base and the first internode of Arabidopsis wildtype and wox4-1 plants

ABSTRACT: Stem samples of wildtype Columbia plants and the wox4-1 mutant (Gabi_462G01) were analyzed in order to draw a connection between general transcriptomic changes during interfascicular formation in the wildtype and WOX4-dependent gene regulation during this process. We used microarrays to identify factors downstream of WOX4 in the formation of the interfascicular cambium.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE24781 | GEO | 2011/09/30

SECONDARY ACCESSION(S): PRJNA132227

REPOSITORIES: GEO

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Expression data from stem samples taken from the base and the first internode of Arabidopsis wildtype and wox4-1 plants

Project description:Stem samples of wildtype Columbia plants and the wox4-1 mutant (Gabi_462G01) were analyzed in order to draw a connection between general transcriptomic changes during interfascicular formation in the wildtype and WOX4-dependent gene regulation during this process. We used microarrays to identify factors downstream of WOX4 in the formation of the interfascicular cambium. Transcriptional profiling, comparing wildtype Columbia and wox4-1 plants. RNA was extracted from 3 biological replicates per plant line and sample type (stem base, first internode), each consisting of a pool of 13-17 stem samples.

2011-09-29 | E-GEOD-24781 | biostudies-arrayexpress

Comparison of Arabidopsis stem base to first internode transcriptional profiles

2010-03-25 | GSE15446 | GEO

Comparison of Arabidopsis stem base to first internode transcriptional profiles

Project description:Subsequently to primary growth, most dicotyledonous plants undergo secondary growth leading to an increased diameter of growth axes. During secondary growth initiation in shoots, a cylindrical meristem, the vascular cambium, is established by the initiation of meristematic activity in interfascicular regions, a process that ultimately leads to the formation of a continuous cylinder of vascular tissue along the shoot axis. In Arabidopsis this happens in a reduced area at the very base of the stem. In this study, a transcriptional comparison between the base and the first internode has been performed in order to asses the physiological state of both sample types and search for potential key regulators of the process. Total RNA was extracted from the base and the first internode from stems of 15cm tall Arabidopsis plants (ecotype Col-0). After RNA amplification, Cy3 or Cy5 targets were produced. Three independent biological replicates were used for each type of sample. Three hybridizations were performed, representing the three independent biological replicates, being one of them a dye swap.

2010-06-05 | E-GEOD-15446 | biostudies-arrayexpress

Expression data from NPA treated stems of Arabidopsis thaliana.

2011-09-01 | GSE24763 | GEO

Expression data from in-vitro induced Interfascicular Cambium of Arabidopsis thaliana

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Expression data from in-vitro induced Interfascicular Cambium of Arabidopsis thaliana

Project description:In-vitro induced establishment and activity of the interfascicular cambium in Arabidopsis thaliana stems under auxin treatments. We used microarrays to detail the global programme of gene expression underlying the establishment and activity of the interfascicular cambium and identified tissue-speciffic up-regulated genes during this process. Different tissue types from in-vitro auxin treated stems were selected at successive stages of cambium establishment using laser capture microdissection for RNA extraction and hybridization on Affymetrix microarrays. We aimed to obtain genes exclussively upregulated in the interfascicular region responsible for cambium establishment and activity.

2010-12-31 | E-GEOD-22947 | biostudies-arrayexpress

Transcriptome profiles and comparison of Arabidopsis thaliana woody (soc1-ful) and WT (Col-0) stems.

Project description:The douple mutant Arabidopsis thaliana soc1 ful, in contrast with WT, produces an interfascicular cambium and a large wood cylinder is the flowering stem. We present the RNAseq data for polyA mRNA of different developmental stages of cambium and wood formation in Arabidopsis thaliana. We sequenced 7 stages; 4 in the woody mutant soc1-6 ful-7 (herbaceous, cambium initiation, wood initiation and leaf) and 3 stages in the WT Col-0 (herbaceous, cambium and leaf). The corresponding stem anatomy is also presented in the manuscript indicating the stage of cambium development and the production of secondary xylem.

2016-05-21 | GSE69167 | GEO

Expression data from 1-N-naphthylphthalamic acid (NPA) treated stems of Arabidopsis thaliana

Project description:In-vivo induced establishment and activity of the interfascicular cambium in Arabidopsis thaliana stems under NPA treatments. We used microarrays to detail the global programme of gene expression underlying the establishment and activity of the interfascicular cambium. 3 mm stem pieces were collected after being in treated with NPA for a week in order to stop the polar auxin transport and induce secondary growth. Collected samples were used for RNA extraction and hybridization on Affymetrix microarrays. We aimed to obtain genes responsible for auxin-induced cambium establishment and activity.

2011-08-31 | E-GEOD-24763 | biostudies-arrayexpress

Arabidopsis thaliana

Project description:Expression data from in-vitro induced Interfascicular Cambium of Arabidopsis thaliana

| PRJNA127793 | ENA

The regulation of cambial activity in Cunninghamia lanceolata involves extensive transcriptome remodeling

Project description:We sequenced mRNA from dormant, reactivating, and actively growing C. lanceolata vascular cambium using tangential cryosections to generate the first transcriptome dynamics that may serve as a gene expression profile blueprint for cambium development and wood formation in the conifer. Examination of mRNA levels in dormant, reactivating, and actively growing C. lanceolata vascular cambium, respectively

2014-03-31 | E-GEOD-37152 | biostudies-arrayexpress

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