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RFX6 maintains gene expression and function of adult human islet ⍺ cells [RNAseq]

ABSTRACT: Transcriptomic profiling by high-throughput sequencing of primary human alpha and beta cells following RFX6 loss of function

ORGANISM(S): Homo sapiens

PROVIDER: GSE248853 | GEO | 2024/01/01

REPOSITORIES: GEO

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RFX6 maintains gene expression and function of adult human islet ⍺ cells [CUT&RUN]

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RFX6 maintains gene expression and function of adult human islet ⍺ cells [RNAseq]

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RFX6 maintains gene expression and function of adult human islet ⍺ cells

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Identification of Rfx6-dependent genes in the adult mouse ileon by RNAseq

Project description:The goal of this experiment was to identify Rfx6-regulated genes in the ileum of adult mice by comparative transcriptome studies, 8 days and 3 months after Rfx6 removal .

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RFX6 maintains gene expression and function of adult human islet ⍺ cells [CUT&RUN]

Project description:RFX6 maintains gene expression and function of adult human islet ⍺ cells [CUT&RUN]

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RFX6 maintains gene expression and function of adult human islet ⍺ cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-01-01 | GSE248854 | GEO

RFX6 haploinsufficiency predisposes to diabetes through impaired beta cell function

Project description:We performed bulk RNA-seq analysis for CRISPR-Cpf1 mutated H1 cells that were differentiated into pancreatic cells. Homozygous mutant was compared to control cells (RFX6-/- vs RFX6+/+) at stage 3 (PF) and stage 5 (EP), and heterozygous mutant was compared to control cells (RFX6-/+ vs RFX6+/+) at stage 7 (SC-islets).

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Transcriptome profile of human pancreatic beta cell line (EndoC-βH2 ) following knockdown of RFX6.

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Rfx6 target genes in the Min6b1 cell line, a pancreatic beta cell model

Project description:Increasing evidence suggests that loss of beta cell characteristics may cause insulin secretory deficiency in diabetes but the underlying mechanisms remain unclear. Here we show that Rfx6, whose mutation leads to neonatal diabetes in man, is essential to maintain key features of functionally mature beta cells in mice. Rfx6 loss in adult beta cells leads to glucose intolerance, impaired beta cell glucose sensing and to defective insulin secretion. This is associated with reduced expression of core components of the insulin secretion pathway including GLucokinase, the Abcc8/SUR1 subunit of KATP channels and voltage-gated Ca2 channels, which are direct targets of Rfx6. Moreover, Rfx6 contributes to the silencing of the vast majority of M-bM-^@M-^\disallowedM-bM-^@M-^] genes, a group usually specifically repressed in adult beta cells, and thus to the maintenance of beta cell maturity. These findings raise the possibility that changes in Rfx6 expression or activity may contribute to beta cell failure in man. Two ChIP-Seq experiments have been performed : 1) anti-HA ChIP-Seq on 3HA-Rfx6 transfected Min6b1 cells and 2) anti-HA ChIP-Seq on Min6b1 cells

2014-12-11 | E-GEOD-62844 | biostudies-arrayexpress

Transcriptome profile of human pancreatic beta cell line (EndoC-M-NM-2H2 ) following knockdown of RFX6.

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