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Hepatic changes after 17 alpha Ethinylestradiol treatment (1D) of male rats

ABSTRACT: Male rat treated with 17-alpha-Ethinylestradiol for 24 hours. Hepatic gene expression changes in comparison with control, excepient treated rats. Keywords: repeat sample

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE2495 | GEO | 2005/05/04

SECONDARY ACCESSION(S): PRJNA91163

REPOSITORIES: GEO

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Hepatic changes after 17 alpha Ethinylestradiol treatment (1D) of male rats

Project description:Male rat treated with 17-alpha-Ethinylestradiol for 24 hours. Hepatic gene expression changes in comparison with control, excepient treated rats.

2010-06-10 | E-GEOD-2495 | biostudies-arrayexpress

Hepatic gene expression after minipump bGH treatment of male rats

Project description:male young rats treated with bGH (minipumps for a week before RNA was prepared from livers Keywords: repeat sample

2005-05-04 | GSE2496 | GEO

Dietary exposure of 17-alpha ethinylestradiol modulates physiological endpoints and gene signaling pathways in female largemouth bass (Micropterus salmoides)

Project description:17alpha-ethinylestradiol (EE2), used for birth control in humans, is a potent estrogen that is found in wastewater at low concentrations (ng/L). EE2 has the ability to interfere with the endocrine system of fish, affecting reproduction which can result in population level effects. The objective of this study was to determine if dietary exposure to EE2 would alter gene expression patterns and key pathways in the liver and ovary and whether these could be associated with reproductive endpoints in female largemouth bass (LMB) during egg development. Female LMB received 70 ng EE2/g feed (feed administered at 1% of body weight) for 60 days. EE2 dietary exposure significantly reduced plasma vitellogenin concentrations by 70%. Hepatosomatic and gonadosomatic indices were also decreased with EE2 feeding by 38.5% and 40%, respectively. Transcriptomic profiling revealed that there were more changes in steady-state mRNA levels in the liver compared to the ovary. Genes associated with reproduction were differentially expressed such as vitellogenin in the liver and aromatase in the gonad. In addition, a set of genes related with oxidative stress (e.g., glutathione reductase and glutathione peroxidase) were identified as altered in the liver and genes associated with the immune system (e.g., complement component 1, and macrophage-inducible C-type lectin) were altered in the gonad. In a follow-up study with 0.2 ng EE2/g feed for 60 days, similar phenotypic and gene expression changes were observed that support these findings with the higher concentrations. This study provides new insights into how dietary exposure to EE2 interferes with endocrine signaling pathways in female LMB during a critical period of reproductive oogenesis.

2011-03-24 | GSE28143 | GEO

Role of AMP-activated protein kinase ?1 in 17?-ethinylestradiol-induced cholestasis in rats.

Project description:Estrogen-induced cholestasis occurs in many women who are susceptible due to pregnancy or hormone replacement therapy for postmenopausal syndrome. 17?-Ethinylestradiol (EE), as a synthetic estrogen, has been widely used to study the underlying mechanisms of estrogen-induced cholestasis. Recent studies have also reported that liver kinase B1 (LKB1)-mediated activation of AMP-activated protein kinase (AMPK) plays a critical role in the regulation of canalicular network formation. However, the role of AMPK in EE-induced cholestasis remains to be determined. In this study, the effects of EE (1-100 µM) on AMPK activation and the expression of farnesoid X receptor (FXR) and hepatic bile acid transporters were examined in in vitro using 3D-cultured rat primary hepatocytes and in in vivo using rat cholestasis models. We also used specific chemical agonist and antagonist of AMPK, AMPK subunit-specific antibodies and lentiviral shRNAs for AMPK?1 and AMPK?2 to delineate the role of AMPK in EE-induced cholestasis and potential cellular mechanisms. We found that EE-induced phosphorylation of AMPK?1 via extracellular signal-regulated kinases-LKB1-mediated signaling pathways and subsequent nuclear translocation accounted for the down-regulation of FXR and bile acid transporters and disruption of bile acid homeostasis. Inhibition of AMPK activation using an AMPK antagonist Compound C (2 µM) or down-regulation of AMPK?1 using gene-specific shRNA attenuated EE-induced cholestasis both in in vitro and in in vivo. In conclusion, these results revealed that activation of cAMP-ERK-LKB1-AMPK?1 signaling pathway plays a critical role in EE-mediated dysregulation of the expression of FXR and bile acid transporters. AMPK?1 may represent an important therapeutic target for estrogen-induced cholestasis.

| S-EPMC5069111 | biostudies-literature

Delayed effect of early-life exposure to 17-alpha-ethinylestradiol on brain proteome of adult mangrove rivulus fish

Project description:PI: Experiments and fish exposures were performed in the laboratory of Frederic Silvestre at the University of Namur (Belgium). Proteomics contact: Proteomics sample preparation and biological mass spectrometry were performed in the laboratory of Prof. Dietmar Kueltz at UC Davis (CA). LC-MS/MS data obtained from brains of 17 adult mangrove rivulus exposed to different doses of 17-alpha-ethinylestradiol during early development.

2018-08-13 | MSV000082814 | MassIVE

Delayed effect of early-life exposure to 17-alpha-ethinylestradiol on the gonad proteome of adult mangrove rivulus fish

Project description:LC-MS/MS data obtained from gonads of 17 adult mangrove rivulus exposed to different doses of 17-alpha-ethinylestradiol during early development. All fish acclimations, treatments, experimental design, and data anlyses were performed in Frederic Silvestre's laboratory at the University of Namur, Belgium by Anne-Sophie Voisin. LC-MS/MS runs were done in Dietmar Kueltz's laboratory at UC Davis.

2018-08-13 | MSV000082816 | MassIVE

Delayed effect of early-life exposure to 17-alpha-ethinylestradiol on the liver proteome of adult mangrove rivulus fish

Project description:LC-MS/MS data obtained from livers of 17 adult mangrove rivulus exposed to different doses of 17-alpha-ethinylestradiol during early development. All fish acclimations, treatments, experimental design, and data anlyses were performed in Frederic Silvestre's laboratory at the University of Namur, Belgium by Anne-Sophie Voisin. LC-MS/MS runs were done in Dietmar Kueltz's laboratory at UC Davis.

2018-08-13 | MSV000082815 | MassIVE

RNA-seq of the zebrafish (danio rerio) brain after developmental exposure to 17-ethinylestradiol

Project description:17-ethinylestradiol (EE2) is a synthetic estrogen commonly used as an active substance in oral contraceptives. It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment. Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non-reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even after a long remediation period. In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0, 2.14 and 7.34 ng/L EE2 from 1 day to 80 days post fertilization. After the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group (0 ng/L) and the 2.14 ng/L group were sampled and 3 female brains from the control group (0 ng/L) and 7.34 ng/L were sampled.

2017-10-02 | E-MTAB-5173 | biostudies-arrayexpress

Gene expression profiles from livers of ethinylestradiol- and chlorpromazine-treated C57BL6/mice after 25 days

Project description:Toxicogenomics is used as a tool to identify mechanisms and markers of cholestasis in C57BL/6 mice treated by oral gavage using ethinylestradiol(EE2) and chlorpromazine (CPZ). A 25 day dose range finding study was performed, which resulted in no changes in clinical chemistry (serum cholesterol, total bile acids, and total bilirubin) or histopathology (hepatocyte vacuolization). Whole genome expression profiling of the liver was assessed after 25 days of repeated exposure with 10 mg/kg bw for CPZ and 3.70 mg/kg bw for EE2.

2017-08-10 | GSE47585 | GEO

Transcription profiling of liver from wild type and ESR1 mutant mice (EAAE/EAAE) after treatment with ethinylestradiol versus vehicle

Project description:To understand the contribution of direct versus indirect DNA binding of estrogen-activated ERa and ERa(EAAE/EAAE) on gene expression in the murine liver, RNA was isolated from the liver of wild-type and ERa(EAAE/EAAE) mice treated perorally with 100µg/kg ethinylestradiol(EE) or vehicle for 4 or 24 hours.

2010-01-28 | E-TABM-739 | biostudies-arrayexpress

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