Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A fluorescent assay for cryptic transcription in Saccharomyces cerevisiae

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Saccharomyces cerevisiae S288C Saccharomyces cerevisiae

PROVIDER: GSE249628 | GEO | 2023/12/08

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

A fluorescent assay for cryptic transcription in Saccharomyces cerevisiae [MNase-seq]

Project description:The disruption of chromatin structure can result in transcription initiation from cryptic promoters within gene bodies. While the passage of RNA polymerase II is a well-characterized chromatin-disrupting force, numerous factors, including histone chaperones, normally stabilize chromatin on transcribed genes, thereby repressing cryptic transcription. DNA replication, which requires a partially overlapping set of histone chaperones, is also inherently disruptive to chromatin, but a role for DNA replication in cryptic transcription has never been examined. In this study, we tested the hypothesis that, in the absence of chromatin-stabilizing factors, DNA replication can promote cryptic transcription in S. cerevisiae. Using a novel fluorescent reporter assay, we show that multiple factors, including Asf1, CAF-1, Rtt106, Spt6, and FACT, block transcription from a cryptic promoter, but are entirely or partially dispensable in G1-arrested cells, suggesting a requirement for DNA replication in chromatin disruption. Collectively, these results demonstrate that transcription fidelity is dependent on numerous factors that function to assemble chromatin on nascent DNA.

2023-12-06 | GSE249469 | GEO

A fluorescent assay for cryptic transcription in Saccharomyces cerevisiae [RNA-seq]

2023-12-07 | GSE249580 | GEO

Fitness of natural Saccharomyces cerevisiae strains

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-07-07 | E-GEOD-49551 | biostudies-arrayexpress

Spore germination in Saccharomyces cerevisiae