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WNT pathway activation promotes phenotypic reprogramming of glioblastoma derived cells in zebrafish nervous system microenvironment

ABSTRACT: phenotypic reprogramming ability of teh zebtafish brain microenviroment on GBM derived cells controlled by the activation of endogenous Wnt pathway

ORGANISM(S): Homo sapiens

PROVIDER: GSE25012 | GEO | 2013/02/28

SECONDARY ACCESSION(S): PRJNA134375

REPOSITORIES: GEO

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WNT pathway activation promotes phenotypic reprogramming of glioblastoma derived cells in zebrafish nervous system microenvironment

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WNT pathway activation promotes phenotypic reprogramming of glioblastoma derived cells in zebrafish nervous system microenvironment

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Multi-inlet spheroid generator for modeling immune microenvironment of glioblastoma (GBM)

Project description:Using the multi-inlet spheroid generator (MSG) platform, we generated stable GBM spheroids and observed their interactions with various immune cells, including microglia and natural killer (NK) cells, at different time points. HMO6 and NK-92 cells were used for microglia and NK cells, respectively. We examined the interactions between tumor-immune cells as well as between different immune cells. The generation of tri-cultured spheroids successfully recapitulated the immunosuppressive microenvironment of the GBM tumor.

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Vitamin E is necessary for zebrafish nervous system development.

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