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Gene expression analysis of four conditions for Puccinia graminis f. sp. tritici

ABSTRACT: Puccinia graminis f. sp. tritici is the cause of wheat stem rust. A microarray was designed from genes predicted from the P. graminis f. sp. tritici genome assembly, and gene expression measured for four conditions which include wheat or barley infecting growth stages initiated by urediniospores. mRNA was prepared from fresh urediniospores, uredinospores germinated for 24 hr, wheat seedlings infected with urediniospores for 8 days, and barley seedlings infected with urediniospores for 8 days. The asexual uredinial infection cycle on wheat produces additional urediniospores, which can start new cycles of wheat infection and are readily spread by aerial transport. This expression data is further described in Duplessis et al, Obligate Biotrophy Features Unraveled by the Genomic Analysis of the Rust Fungi, Melampsora larici-populina and Puccinia graminis f. sp. tritici

ORGANISM(S): Puccinia graminis f. sp. tritici

PROVIDER: GSE25020 | GEO | 2010/12/31

SECONDARY ACCESSION(S): PRJNA134391

REPOSITORIES: GEO

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Gene expression analysis of four conditions for Puccinia graminis f. sp. tritici

Project description:Puccinia graminis f. sp. tritici is the cause of wheat stem rust. A microarray was designed from genes predicted from the P. graminis f. sp. tritici genome assembly, and gene expression measured for four conditions which include wheat or barley infecting growth stages initiated by urediniospores. mRNA was prepared from fresh urediniospores, uredinospores germinated for 24 hr, wheat seedlings infected with urediniospores for 8 days, and barley seedlings infected with urediniospores for 8 days. The asexual uredinial infection cycle on wheat produces additional urediniospores, which can start new cycles of wheat infection and are readily spread by aerial transport. This expression data is further described in Duplessis et al, Obligate Biotrophy Features Unraveled by the Genomic Analysis of the Rust Fungi, Melampsora larici-populina and Puccinia graminis f. sp. tritici A total of 12 samples were analyzed, including three biological replicates of the four conditions.

2010-12-31 | E-GEOD-25020 | biostudies-arrayexpress

Transcription analysis by RNAseq of adult wheat after inoculation with Puccinia graminis f. sp. tritici

Project description:The RNA sequencing analysis was undertaken to investigate the transcriptomic changes in adult wheat inoculated with Puccinia graminis f. sp. tritici the causal agent of stem rust disease. The project firstly aims to compare gene expression in one susceptible wheat line with two wheat lines exhibiting adult plant resistance to the stem rust. Secondly, the project aims to examine the temporal changes in gene expression in wheat after inoculation. Wheat plants was grown until maturity under greenhouse conditions. Plants were inoculated with Puccinia graminis f. sp. tritici and the flag leaf sheath sampled for RNA sequencing. The project aims to give essential insight into the adult plant resistance response in wheat to Puccinia graminis f. sp. tritici inoculation.

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Transcriptome-wide Association study identifies putative Elicitors/Suppressor of Puccinia graminis f. sp. tritici that Modulate Barley rpg4-mediated Stem Rust Resistance

Project description:Transcriptomic profiling was done on barley samples incoulated with different Puccinia graminis f. sp. tritici (Pgt) isolates of varying virulence profile on barley stem rust resistance gene rpg4/5 and Rpg1. Several differentally expressed host and pathogen genes were identified while comparing gene expression profiles between group of samples inoculated with isoaltes of varying virulence. Several variants with genes of pathogen were idnetified that are possibly associated with virulence to rpg4/5.

2019-04-30 | GSE130423 | GEO

A three-dimensional chromatin contact map of Puccinia graminis f.sp. tritici

Project description:Puccinia graminis f.sp. tritici (Pgt), the causal agent of stem rust disease in wheat, is one of the most destructive pathogens and can cause severe yield losses. Here, we utilize Hi-C sequencing technology to scaffold and phase the haplotypes for the genome assembly of a US Pgt isolate 99KS76A-1.

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Inoculation of rice with the non-host pathogen Puccinia triticina f. sp. tritici elicits an active transcriptional response

Project description:Japonica rice cultivar Nipponbare was inoculated with wheat leaf rust (Puccinia triticina f. sp. tritici, non-host pathogen to rice) to compare gene expression profiles with mock-inoculated controls. Although eventually failed in invasion, leaf rust induced a set of rice genes that were distinctally up-regulated, some of those were confirmed by quantitative real-time PCR assays.

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Wheat Transcriptome

Project description:Stripe rust (Puccinia striiformis f. sp. tritici; Pst) and powdery mildew (Blumeria graminis f. sp. tritici; Bgt) are important diseases of wheat (Triticum aestivum) worldwide. Similar mechanisms and gene transcripts are assumed to be involved in the host defense response because both pathogens are biotrophic fungi. The main objective of our study was to identify co-regulated mRNAs that show a change in expression pattern after inoculation with Pst or Bgt, and to identify mRNAs specific to the fungal stress response. In the present study, cDNA libraries were constructed from leaves inoculated with Pst or Bgt at 0, 1, 2 and 3 days post-inoculation (dpi) with three biological replicates, and then sequenced using the Illumina HiSeq™ 2000 platform. Note: Samples in SRA were assigned the same sample accession. This is incorrect as there are different samples, hence “Source Name” was replaced with new values. Comment[ENA_SAMPLE] contains the original SRA sample accessions.

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Transcriptional profiling reveals no response of fungal pathogens to the durable, quantitative Lr34 disease resistance gene of wheat

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2018-07-07 | GSE100904 | GEO

Susceptible wheat cultivar infected with the wild type Puccinia graminis (isolate 99KS76A-1) and its mutants carrying knockout mutations in AvrSr35.

Project description:The assess the effect of AvrSr35 knock-out on host-pathogen interaction in the compatible host, we have performed time-course analysis of leaf transcriptomes obtained by infecting susceptible wheat cultivar Fielder with the wild type Puccinia graminis f.sp. tritici (Pgt) isolate 99KS76A-1 and its three mutants, M1, M4 and M7, that carry loss-of-function mutations in the AvrSr35 gene.

2017-11-02 | GSE106397 | GEO

RNA sequencing of stripe rust infected wheat leaves and isolated haustoria

Project description:The fungus Puccinia striiformis f.sp. tritici (PST) is the causal pathogen of stripe rust in wheat. New highly virulent PST races appeared at the beginning of this century and spread rapidly causing significant yield losses in wheat production worldwide. Race PST-08/21 was isolated in the UK in 2008 Yr1, Yr2, Yr3, Yr4, Yr6, Yr9, Yr17, Yr27, Yr32, YrRob, YrSol. We applied the RNAseq approach to refine the gene prediction in de novo assembled PST 08/21 contigs and to determine which genes are expressed during wheat infections. Total RNA was extracted from a pool of stripe rust infected wheat leaves and from two biological replicates of haustoria isolates.

2013-08-19 | E-GEOD-42496 | biostudies-arrayexpress

Rice gene global expression during nonhost interaction with Blumeria graminis f. sp. hordei (Bgh)

Project description:Powdery mildew is a very common plant disease and only few plants are immune. Host interactions have been identified and characterized for the pathosystems barley-B. graminis f. sp. tritici (Bgt) and wheat-B. graminis f. sp. hordei (Bgh), whereas no data are reported about powdery mildew and nonhost plants, such as rice. On the other hand rice nonhost resistance is widely unexploited and only few expression data are available. To characterize rice response during nonhost interaction with Bgh, a global expression analysis was performed by using the GeneChip® Rice Genome Array. To describe rice gene expression profiles during nonhost interaction, 2 week-old rice plantlets were inoculated with Bgh. Treated (inoculated) and control (mock) samples were collected 24 hours post-inoculation for GeneChip® Rice Genome Array hybridization.

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