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Glutaminase-1 Inhibition Alleviates Senescence of Wharton’s Jelly-derived Mesenchymal Stem Cells via Senolysis.

ABSTRACT: we attempted to identify the genetic changes in the remaining MSCs after BPTES treatment and identified senescence-related candidate genes that recovered after BPTES treatment. The transcriptomes of U-MSCs and rS-MSCs were analyzed to determine the genetic significance of replicative senescence in WJ-MSCs

ORGANISM(S): Homo sapiens

PROVIDER: GSE250218 | GEO | 2024/09/26

REPOSITORIES: GEO

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Glutaminase-1 Inhibition Alleviates Senescence of Wharton’s Jelly-derived Mesenchymal Stem Cells via Senolysis.

Project description:Glutaminase-1 Inhibition Alleviates Senescence of Wharton’s Jelly-derived Mesenchymal Stem Cells via Senolysis.

| PRJNA1052687 | ENA

Comparison of human isogeneic Wharton’s Jelly MSCs and iPSC-derived MSCs responses to interferon-gamma (IFNG) stimulation

Project description:To explore differences between native MSCs (nMSCs) and iPSC-derived MSCs (iMSCs), we developed isogeneic lines from Wharton’s Jelly (WJ) from the umbilical cords of two donors (#12 and #13) under xeno-free conditions. Next, we reprogrammed them into iPSCs (iPSC12 and iPSC13) and subsequently differentiated them back into iMSCs (iMSC12 and iMSC13) using two different protocols, which we named ARG and TEX. As an additional control, we used human embryonic stem cell line KCL034 to differentiate the cells into eMSC following ARG and TEX protocols. We assessed IFNG-induced changes in all lines. Our data suggest that, following a careful selection and screening of donors, nMSCs from umbilical’s cord WJ can be easily reprogrammed into iPSCs providing an unlimited source of material for differentiation into iMSCs. However, the differentiation protocol should be chosen depending on their clinical use.

2020-03-23 | GSE131499 | GEO

Gene expression patterns of mesenchymal stem cells from bone marrow and Wharton's jelly of umbilical cord

Project description:Mesenchymal stem cells (MSCs) are promising tools for the treatment of diseases such as infarcted myocardia and strokes because of their ability to promote endogenous angiogenesis and neurogenesis via a variety of secreted factors. MSCs found in the Wharton's jelly of the human umbilical cord are easily obtained and are capable of transplantation without rejection. We isolated MSCs from WhartonM-bM-^@M-^Ys jelly and bone marrow (WJ-MSCs and BM-MSCs, respectively) and compared their transcriptional profiles. In this study, BM-MSCs and WJ-MSCs were isolated from 4 and 3 independent donors, respectively.

2013-09-30 | E-GEOD-48022 | biostudies-arrayexpress

Anti-necroptotic effects of human Wharton’s jelly-derived mesenchymal stem cells in skeletal muscle cell death model via secretion of GRO-α

Project description:Human mesenchymal stem cells (hMSCs) have therapeutic applications and potential for use in regenerative medicine. However, the use of hMSCs in research and clinical medicine is limited by a lack of information pertaining to their donor-specific functional attributes. In this study, we compared the characteristics of same-donor derived placenta (PL) and Wharton’s jelly (WJ)-derived hMSCs, we also compared their mechanism of action in a skeletal muscle disease in vitro model. The effects of GRO-α were also investigated in a Duchenne muscular dystrophy (DMD) mouse model. The same-donor-derived hWJ- and hPL-MSCs exhibited typical hMSC characteristics. GRO-α was differentially expressed in hWJ- and hPL-MSCs. However, when the secretion of GRO-α was considered, hWJ-MSCs displayed a higher ability to inhibit necroptosis in skeletal muscle cells than hPL-MSCs. This demonstrates the anti-necroptotic therapeutic effect of GRO-α in the skeletal muscle cell death model. Furthermore, considering their potential to inhibit necroptosis in skeletal muscle cells, hWJ-MSCs and the derived GRO-α are novel treatment options for skeletal muscle diseases such as DMD.

2024-09-25 | GSE277126 | GEO

mRNA expression profile of diesel exhaust particle treated human Wharton’s jelly-derived mesenchymal stem cells

Project description:We performed RNA sequencing to identify differentially expressed gene patterns between WJ-MSC treated with or without 5 µg/mL of DEP.

2021-03-25 | GSE169533 | GEO

Gene expression profile of Wharton-jelly mesenchymal stem cells at 12th in vitro expansion cycle compared to Wharton-jelly mesenchymal stem cells at 4th in vitro expansion cycle

Project description:Mesenchymal stem cells (MSCs) are generally considered the main tool box for cell-based therapies and compared to embryonic stem cells (ESCs), MSCs exhibit many advantages. It has been shown that UC matrix (the Wharton’s Jelly surrounding umbilical vessels) contains a great number of mesenchymal cells. The abundant amount of Wharton’s Jelly (WJ) makes it an attractive source of MSCs for cell-based therapies. recently have been highlighted the changes in protein expression profiling during in vitro expansion of WJ-MSC, probably related to the gradual reduction of their staminal plasticity and to the biological cellular mechanism occurring in the cellular aging. The aim of the present work was to analyze the transcriptomic profile useful to the definition of WJ-MSC characteristics and therefore identify possible different new marker candidates. In these contest, changes in gene expression profiles occurring during WJ-MSC in vitro growth wile by analyzed in order to find possible modulation related to their long expansion and fast growth abilities. We analyzed the changes in total RNA expression at the 12th in vitro expansion cycle compared to the 4th in vitro expansion cycle.

2012-06-28 | GSE34929 | GEO

Extracellular Vesicles Derived from Wharton’s Jelly Mesenchymal Stem Cells Inhibit the Tumor Environment via the miR-125b/HIF-1a Signaling Pathway

Project description:Purpose: small RNAseq analyses were conducted to identify the key miRNAs which involves in the regulation of tumor development in the Wharton's Jelly Mesehchymal Stem Cell-derived EV (WJ-EV) treated breast cancer cell (BCC) Methods: MDAMB231, a triple negative breast cancer cell line, were treated with WJ-EV at 0 and 24 hours, then the cell samples were harvested at 48 hours. The cells were cultured under a hypoxic condition (1%O2). Results: 1169 miRNAs were identified in original BCC and WJ-EV treated BCC (pBCC), respectively.

2022-08-24 | GSE197863 | GEO

Stem Cells: Umbilical Cord/Wharton’s Jelly Derived

Project description:As a commonly discarded tissue, the umbilical cord contains a rich source of mesenchymal stromal cells, which are therefore obtained non-invasively. As a perinatal population, replicative senescence is delayed and cell expansion is expedited, enabling collection of many clinically relevant doses from a single donor cord at low passage numbers. In this chapter, we will discuss the structure of the umbilical cord and the various stromal populations contained within that have been described. We also highlight the lack of consensus on both anatomical descriptors of the cord tissue, and standardized isolation techniques for these different populations, which together with insufficient methodological transparency may be hampering progress within the field. We then review the basic and preclinical models of disease that have been targets of umbilical cord-derived mesenchymal stromal cells. Finally, we close with a discussion of their use in clinical trials.

| S-EPMC7992171 | biostudies-literature

Gene expression patterns of mesenchymal stem cells from bone marrow and Wharton's jelly of umbilical cord

Project description:Mesenchymal stem cells (MSCs) are promising tools for the treatment of diseases such as infarcted myocardia and strokes because of their ability to promote endogenous angiogenesis and neurogenesis via a variety of secreted factors. MSCs found in the Wharton's jelly of the human umbilical cord are easily obtained and are capable of transplantation without rejection. We isolated MSCs from Wharton’s jelly and bone marrow (WJ-MSCs and BM-MSCs, respectively) and compared their transcriptional profiles.

2013-09-30 | GSE48022 | GEO

Extracellular Vesicles Derived from Wharton’s Jelly Mesenchymal Stem Cells Inhibit Inflammatory Responses to SARS-CoV-2 and Influenza Viral Proteins via the miR-146a/ NF-κB pathway

Project description:Purpose: small RNAseq analyses were conducted to identify the key miRNAs which involves in the suppression of inflammatory respons via NF-kB pathway in the Wharton's Jelly Mesehchymal Stem Cell-derived EV (WJ-EV). Methode: WJ-MSC were cultured using EV-Depleted medium for 24 hr. for hWJ-EV, after reach 80% confluences the cells were cultured under a hypoxic condition (1%O2) for 48 hr. The conditioned medium from WJ-MSC and hWJ-MSC then were collected and centrifuged to get the EVs. Result: miR-21, miR-143, and miR-146a were involved in the regulation of the NF-κB signaling pathway and inflammatory cytokines.

2024-12-01 | GSE273241 | GEO

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