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SciMET-cap: High-throughput single-cell methylation analysis with a reduced sequencing burden

ABSTRACT: DNA methylation is a key component of the mammalian epigenome, playing a regulatory role in development, disease, and other processes. Robust, high-throughput single-cell DNA methylation assays are now possible (sciMET); however, the genome-wide nature of DNA methylation results in a high sequencing burden per cell. Here, we leverage target enrichment with sciMET to capture sufficient information per cell for cell type assignment using substantially fewer sequence reads (sciMET-cap). Sufficient off-target coverage further enables the production of near-complete methylomes for individual cell types. We characterize sciMET-cap on human PBMCs and brain (middle frontal gyrus).

ORGANISM(S): Homo sapiens

PROVIDER: GSE250282 | GEO | 2023/12/19

REPOSITORIES: GEO

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