MEK5 is Activated by Shear Stress, Activates ERK5 And Induces KLF4 To Modulate TNF Responses in Human Dermal Microvascular Endothelial Cells
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
PROVIDER: GSE25145 | GEO | 2010/12/31
SECONDARY ACCESSION(S): PRJNA134487
REPOSITORIES: GEO
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AP-MS studies:
For KLF gain-of-function, pulmonary artery endothelial cells (PAEC) were transduced with adenoviral vectors encoding a constitutively active mutant of MEK5 (caMEK5), or GFP as control. 90h post-transduction cells were lysed and AP was performed using antibodies targeting either KLF (sc166238, Santa Cruz Biotechnology) or FLAG (F7425, Millipore Sigma).
Datafiles correspond to:
GK: GFP-tranduced controls using anti-KLF antibodies for AP.
GF: GFP-tranduced controls using anti-FLAG antibodies for AP.
KK: caMEK5-transduced cells with anti-KLF antibodies for AP.
KF: caMEK5-tranduced cells with anti-FLAG antibodies for AP.
2020-07-24 | MSV000085825 | MassIVE