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Changes in gene expression in MKN45 cells in response to Helicobacter pylori lipopolysaccharide

ABSTRACT: This study set out to identify global changes in gene expression in MKN45 gastric epithelial cells following 8 hours stimulation with 10 μg/ml lipopolysaccharide (LPS) from the gastric pathogen H. pylori. Microarray analysis was used to compare changes in gene expression between cells treated with 10 μg/ml H. pylori LPS and untreated cells at the same time point.

ORGANISM(S): Homo sapiens

PROVIDER: GSE25147 | GEO | 2011/01/12

SECONDARY ACCESSION(S): PRJNA142771

REPOSITORIES: GEO

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Changes in gene expression in MKN45 cells in response to Helicobacter pylori lipopolysaccharide

Project description:This study set out to identify global changes in gene expression in MKN45 gastric epithelial cells following 8 hours stimulation with 10 μg/ml lipopolysaccharide (LPS) from the gastric pathogen H. pylori. Microarray analysis was used to compare changes in gene expression between cells treated with 10 μg/ml H. pylori LPS and untreated cells at the same time point. Total RNA was harvested from MKN45 cells following treatment with LPS for 8h or untreated cells at the same time-point. 2 independent experiments were carried out. RNA was labeled and hybridized to GeneChips to analyse changes in gene expression.

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