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Nutrigenomics in honey bees: the impact of pollen nutrients on bee health

ABSTRACT: We studied the molecular mechanisms underlying the impact of pollen nutrients on honey bee (Apis mellifera) health and how those nutrients improve resistance to parasites. Using digital gene expression, we determined the changes in gene expression induced by pollen intake in worker bees parasitized or not by the mites Varroa destructor, known for suppressing immunity and decreasing lifespan of bees.

ORGANISM(S): Apis mellifera

PROVIDER: GSE25161 | GEO | 2011/11/06

SECONDARY ACCESSION(S): PRJNA134353

REPOSITORIES: GEO

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Nutrigenomics in honey bees: the impact of pollen nutrients on bee health

Project description:We studied the molecular mechanisms underlying the impact of pollen nutrients on honey bee (Apis mellifera) health and how those nutrients improve resistance to parasites. Using digital gene expression, we determined the changes in gene expression induced by pollen intake in worker bees parasitized or not by the mites Varroa destructor, known for suppressing immunity and decreasing lifespan of bees. bees with or without verroa, and fed or not fed pollen

2011-11-06 | E-GEOD-25161 | biostudies-arrayexpress

Antennae transcriptomics of Varroa-hygienic and Nurse/Forager bees

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Project description:In this study we addressed whether the transcriptome profile in the honey bee brain is similar for two major parasites of honey bee, Varroa destructor and Nosema ceranae. Honey bees parasitized by these two parasites show accelerated behavioral maturation and deficiences in orientation and learning/memory that we hoped to characterized at the transcriptomic level. honey bee adults infested by Varroa destructor or Nosema ceranae compared to control bees, in duplicate

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Brain transcriptomics of honey bee parasitized by Varroa destructor or Nosema ceranae

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Diversity of populations of Deformed wing virus in the honeybee (Apis mellifera) pupae overtly infected by injection of DWV virus preparations from overtly and covertly infected honeybees to haemolymph.

Project description:Experiment was designed (i) to analyse the strain composition of Deformed wing virus (DWV) populations in covertly and overtly infected honeybees (Apis mellifera) from Varroa-free and Varroa-infested colonies, and (ii) to determine abundance of the DWV strains following direct injection of the DWV preparations from covertly and overtly infected bees to the bee pupae haemolymph in the absence of Varroa destructor mites. Experiment included isolation of DWV preparations from the following bees: covertly-infected bees from Varroa-free colony, covertly infected bees exposed orally to the Varroa-selected DWV strains, and the overtly infected Varroa-exposed bees. Honeybee pupae were experimentally injected with those DWV preparations and sampled 4 days post injection following development of overt DWV infection. A series of the DWV cDNA fragment covering complete DWV genomic RNA sequences were amplified by RT-PCR using RNA extracted from virus preparations and the injected pupae. The cDNA preparations were sequenced using next generation(Illumina HighSeq 2000) paired-end sequencing to obtain data on the DWV strain composition.

2013-09-01 | E-MTAB-1675 | biostudies-arrayexpress

Transcriptome of Varroa destructor life cycle

Project description:To study the underlying molecular mechanisms during the Varroa destructor life cycle, we carried out transcriptomic profiling of seven stages: young mites (collected from P8 to P9 brood cells), phoretic mites (collected on adult bees), arresting mites (collected in unsealed L5 brood cells), pre-laying mites (collected from sealed brood cells containing moving larva), laying mites (collected from sealed brood cells containing pre-pupae), post-laying mites (collected from capped brood cells containing purple-eye and white-body pupae P5), emerging mites (collected from P8 to P9 brood cells). In addition, we sampled non-reproducing mites (collected from P5 brood cells, but without offspring), males (collected from P8 to P9 brood cells), and phoretic mites artificially reared in cages with adult bees. This study was performed using Apis mellifera L. honey bee colonies naturally infested by Varroa destructor mites. Adult mites were collected from 4 unrelated colonies.

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2013-06-01 | E-MTAB-1285 | biostudies-arrayexpress

Effects of Deformed wing virus infection and the mite Varroa destructor on siRNA and miRNA composition in the honeybee (Apis mellifera)

Project description:Experiment was designed to study the effect of Deformed wing virus (DWV) and the mite Varroa destructor on on siRNA and miRNA composition using high-throughput sequencing of small RNA in developing worker honeybee (Apis mellifera). Newly hatched bee larvae (day 3 of bee development) were transferred from a Varroa-free colony with low DWV levels to a Varroa-infested colony with high levels of DWV in bees and Varroa mites. All transferred larvae were receiving the DWV strains present in this Varroa-infested colony with the food delivered by the nurse bees until their capping (day 8). About half of these larvae were capped with Varroa mite and were subjected to the mite piercing and feeding on their haemolymph during pupal development until sampling at purple eye stage (day 14). Exposure to the mite piercing and feeding resulted in about 1000-fold increase of the DWV levels in the majority of the mite-exposed pupae compared to the control pupae and the pupae not exposed to Varroa mites.

2013-09-01 | E-MTAB-1671 | biostudies-arrayexpress

Omics-based analysis of honey bee (Apis mellifera) response to Varroa sp. interaction reveals changes impairing the winter bee generation

Project description:Extensive annual losses of honey bees (Apis mellifera L.) represent a global problem for agriculture and biodiversity. The parasitic mite Varroa destructor in association with viral co-infections plays a key role in this phenomenon; however, the precise mechanisms are still unclear. We employed a unique combination of transcriptomic, proteomic, metabolomic, and functional analyses to elucidate the effects of Varroa parasitisation. We focused on complex differences between parasitised and unparasitised ten-days old honey bee workers collected from identical colonies before overwintering. Honey bees exposed to mite parasitation during their development revealed alterations in transcriptome and proteome related to immunity, oxidative stress, olfactory recognition, metabolism of sphingolipids and RNA regulatory mechanisms. Specifically, immune reactions and sphingolipids metabolism were strongly up-regulated in parasitised honey bees; whereas olfactory recognition and oxidative stress pathways were down-regulated compared to unparasitised bees. Additionally, the metabolomic analysis confirmed the depletion of nutrients, decreased energy stores and generally disrupted metabolism of parasitised workers, as previously reported. By virtue of comprehensive omics-based analysis, we define the key changes in the honey bee facing Varroa parasitism and suggest possible mechanisms underlying its detrimental effects. This study provides a theoretical basis for future efforts in efficient control strategies against Varroa mites.

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