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Effects of high cholesterol diet on sperm small RNA expression profile utilizing two different RNA-seq methods

ABSTRACT: Purpose: To reveal the small RNA profiles in the sperm using traitional and PANDORA-seq methods Methods: 3 week old LDLR-/- male mice were fed a high cholesterol or low cholesterol diet for 9 weeks. Sperm RNA was extracted via Trizol. Results: paternal HCD feeding can lead to sperm tsRNA/rsRNA landscape changes that are undetected by traditional RNA-seq

ORGANISM(S): Mus musculus

PROVIDER: GSE251712 | GEO | 2024/10/08

REPOSITORIES: GEO

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Effects of high cholesterol diet on intimal small RNA expression profile utilizing two different RNA-seq methods [Intima_small_RNA]

Project description:Purpose: To reveal the small RNA profiles in the intima using traditional and PANDORA-seq methods. Methods: 3-week-old LDLR-/- male mice were fed a high cholesterol or low cholesterol diet for 9 weeks. Intimal RNA was extracted by injecting Trizol into the aorta and collecting the flow through for further processing. Results: PANDORA-seq revealed an abundant amount of tsRNA and rsRNA that were undetected by traditional-seq. Importantly, PANDORA-seq revealed that many differentially regulated sncRNAs also undetected with traditional-seq.

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Hyperlipidemia elicits an IFN-based CD4+ T cell response

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Effects of high cholesterol diet on sperm small RNA expression profile utilizing two different RNA-seq methods

Project description:Effects of high cholesterol diet on sperm small RNA expression profile utilizing two different RNA-seq methods

| PRJNA1055102 | ENA

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