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Pig Taste Cell-Derived Organoids Synthesize Insulin

ABSTRACT: Previously we showed that taste receptor cells in situ in taste buds synthesize insulin. Here we describe a model of pig taste organoid culture in which we have promoted insulin expression by induction of quiescence. The cellular heterogeneity of the lingual epithelium is maintained in the organoids, and stem cell type and organoid architecture can be controlled through changes in media composition and/or use of static versus dynamic culture. Pig taste organoids were maintained long term and organoids cultured in low sheer stress dynamic exhibited an architecture and expression profile akin to the native tissue. Porcine taste organoids also contained insulin, and the insulin critical transcription factors MAFA and PAX4. These results provide a pig model of taste organoid culture that can be used universally and bring us closer to the use of the taste tissue as a new renewable source of beta cells

ORGANISM(S): Sus scrofa

PROVIDER: GSE252240 | GEO | 2024/01/31

REPOSITORIES: GEO

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Pig Taste Cell-Derived Organoids Synthesize Insulin

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