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METABOLIC INSIGHTS INTO DIVERSION OF HYDROPHOBIC AND HYDROPHILLIC WASTE TOWARDS OMEGA FATTY ACID IN THRAUSTOCHYTRIDS

ABSTRACT: Here, we investigated marine thraustochytrid Schizochytrium limacinum SR21 for its ability to convert waste oil, mixture of commercial oils (mCOs) and volatile fatty acids i.e., acetic acid and butyric acid into ω-3 fatty acid; docosahexaenoic acid (DHA). Metabolic insights through whole cell transcriptomic aid in tracing the route of substrate assimilation.

ORGANISM(S): Aurantiochytrium limacinum

PROVIDER: GSE252403 | GEO | 2024/04/10

REPOSITORIES: GEO

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METABOLIC INSIGHTS INTO DIVERSION OF HYDROPHOBIC AND HYDROPHILLIC WASTE TOWARDS OMEGA FATTY ACID IN THRAUSTOCHYTRIDS

Project description:METABOLIC INSIGHTS INTO DIVERSION OF HYDROPHOBIC AND HYDROPHILLIC WASTE TOWARDS OMEGA FATTY ACID IN THRAUSTOCHYTRIDS

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RNAseq of Salmo salar parrs with CRISPR/Cas9 induced knockout in the elovl2 gene versus wild type

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Project description:This study considers the physiological modulation of liver proteins due to the supplementation with fish oils under two different dietary backgrounds: low- or high- fat and sucrose diets, and the effect of their combination with an antioxidant agent (grape polyphenols) which provides reducing power. For this scope, a quantitative proteomics approach based on the Isobaric Tag for relative and Absolute Quantitation methodology (iTRAQ)-coupled to nano-LC-MS/MS and complemented with 2D-DIGE analysis were used for determining the regulation of liver proteins exerted by the supplementation with fish oils, polyphenols or their combination of Wistar Kyoto rats in the two chosen dietary backgrounds. This experimental design was useful to investigate if the behavior of fish oils changes when the dietary background is modified and the possible synergy between fish oils and polyphenols. Results show that the capacity of fish oils, polyphenols or their combination for down or up-regulating liver proteins depends on the dietary context. In the background of low-fat low-sucrose healthy diets, 10 different proteins were altered by the sum of three supplements, in opposite to the 45 altered proteins found in the high-fat high-sucrose unhealthy diets. In both situations, fish oils seemed to be the main force for regulating liver proteins, although the addition of polyphenols was able to modulate some fish oils effects. Moreover, we provide evidence of the effect of fish oils and their combination with grape polyphenols for improving biochemical parameters and for reducing enzymes of hepatic lipogenesis and glycolysis, for enhancing fatty acid beta oxidation and insulin signaling and for the amelioration of endoplasmic reticule stress and protein oxidation when are included in an unhealthy diet.

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