Dataset Information

Human Platelet Lysate-Cultured Adipose-Derived Stem Cell Sheets Promote Angiogenesis and Accelerate Wound Healing via CCL5 Modulation

ABSTRACT: To further differentiate the gene expression of HPL-cultured or FBS-cultured ASC sheets, we have employed microarray analysis. ASCs were isolated from the subcutaneous fat tissue of four non-diabetic, non-smoking female donors with an average age of 45 y (32–57 y) and an average body mass index of 24.6 (21.0–26.6). Significantly up-regulated or down-regulated genes are highlighted in the HPL-cultured ASC sheets. Pathway enrichment analysis showcasing the top 10 hallmark gene-sets from MSigDB significantly enriched in up-regulated (top) or down-regulated (bottom) genes. The heatmap represented the expression patterns of angiogenesis-related genes between the HPL-cultured and FBS-cultured ASC sheets.

ORGANISM(S): Homo sapiens

PROVIDER: GSE252798 | GEO | 2024/06/13

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Purpose: The population of muscle-derived stem cells called MuStem cells is presented as promising candidate for cell-based therapy of muscle diseases. To validate if this agent can be really presented as therapeutic product and so to be eligible to a future clinical use, it is now required to demonstrate beforehand an efficacy with cells prepared in compliance with good manufacturing practices (GMPs). The aim of the current study was to evaluate the use of two xeno-free blood derivatives corresponding to human serum (HS) and human platelet lysate (hPL) as alternatives to controverted but until now used fetal bovine serum (FBS) for isolation and expansion of human MuStem (hMuStem) cells. Methods: A comparative study was performed with hMuStem cells isolated and in vitro expanded by using commercially available HS and hPL to determine its impact on their proliferation rates, clonogenicity, myogenic commitment level and oligopotency with regard to results obtained under FBS-based medium. Also, their respective phenotype and global gene expression patterns were investigated by flow cytometry and high throughput 3’ digital gene expression RNA-sequencing in order to define a possible differential impact of the human nutrients tested. Results: Comparatively to FBS-based medium, use of HS- and hPL-supplemented ones efficiently supported long-term proliferation of hMuStem cells and enhanced clonogenicity, without main modification of their expression profile and allowing besides limiting the supplementation in growth factors. In vitro differentiation assay combined to transforming growth factor β1 (TGF-β1)-depletion experiments showed a lower myogenic commitment level as well as fusion ability of hMuStem cells when cultured with hPL-based medium according to a TGF-β1-independent process. Use of hPL-derived 3D hydrogel or fibrinogen-depleted hPL demonstrated that heparin-free hPL derivatives maintain consequent myogenic differentiation potential. In addition, the reduced myogenicity was shown to be rapidly reversible following replacement of hPL by HS or fibrinogen-depleted hPL. Conclusions: All together, our original findings position HS and hPL as efficient and suitable alternatives to FBS for preparation of hMuStem cell batch in compliance with GMPs.

Dataset Information

Human Platelet Lysate-Cultured Adipose-Derived Stem Cell Sheets Promote Angiogenesis and Accelerate Wound Healing via CCL5 Modulation

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets