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Single-cell transcriptome and TCR sequencing reveals immune cell heterogeneity in the submandibular gland of SS mice

ABSTRACT: In this study, we analyzed the submandibular gland and submandibular gland-draining lymph nodes of P40-/-CD25-/- (SS mice) and P40-/-CD25+/- (control mice) using single-cell transcriptome and TCR sequencing of leukocytes. A total of 24,115 cells were obtained for analysis, of which 9,897 cells were from the submandibular gland and 14,218 cells were from the draining lymph nodes. We found that tissue-resident CD8+T cells (CD8+Trm) in the submandibular gland of SS mice expanded massively and highly expressed PD-1. These CD8+Trm cells, which highly express PD-1, exhibit clonal expansion and cytotoxicity. In addition, shared TCR analysis found that potential CD8+Trm precursor cells in the draining lymph nodes exhibited a Gzmk+Pdcd1+ phenotype. In summary, we found that PD-1+CD8+Trm cells in the submandibular gland of SS mice are potentially pathogenic and their precursors also express PD-1.

ORGANISM(S): Mus musculus

PROVIDER: GSE253042 | GEO | 2024/12/15

REPOSITORIES: GEO

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