Transcriptomics

Dataset Information

0

Adenoviral vector transduced in vitro differentiated C57bl6 BMDM cells


ABSTRACT: Total mRNA was extracted at 48 hour post transduction using Qiagen RNeasy Kits following the manufacture’s protocol.1 ug ofRNAwas used for cDNA library construction and bulk RNA sequencing were performed by Novogene (Sacramento CA). RNA-seq raw data were analysised using Partek Flow. Briefly, sequencing reads were aligned to the mouse or human genome using Spliced Transcripts Alignment to a Reference (STAR 2.5.3a). Aligned reads were then quantified to transcriptome (mm10; Ensembl Transcripts release 100), and normalized (Median Ratio normalization). Differentiated gene expression analysis (DESeq2) were performed to generate raw counts

ORGANISM(S): Mus musculus

PROVIDER: GSE253342 | GEO | 2024/04/21

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2019-04-01 | GSE127171 | GEO
| EGAD00010001703 | EGA
2021-06-23 | GSE178685 | GEO
2013-12-20 | GSE50063 | GEO
2019-05-31 | GSE122900 | GEO
2021-04-30 | GSE162254 | GEO
2013-12-20 | E-GEOD-50063 | biostudies-arrayexpress
2017-06-29 | GSE98388 | GEO
2017-08-17 | GSE100277 | GEO
2018-03-22 | GSE112162 | GEO