Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of L1TD1-associated transcripts in DNMT1 KO HAP1 cells

ABSTRACT: In this study, we aimed at determining RNA interactors of L1TD1 RNPs. To achieve this, DNMT1 KO and DNMT1 L1TD1 DKO HAP1 cells were generated by Crispr/Cas9 gene editing tool and L1TD1 RIP was performed by using these cell lines. Our study reveals a comprehensive analysis of differentially enriched transcripts in L1TD1 containing RNPs.

ORGANISM(S): Homo sapiens

PROVIDER: GSE254459 | GEO | 2025/01/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Expression profile of DNMT1 KO and DNMT1 L1TD1 HAP1 cells by high throughput sequencing

Project description:In this study, we aimed at analyzing gene expression profile of DNMT1 KO and DNMT1 L1TD1 DKO HAP1 cells using high throughput sequencing. Our study reveals a comprehensive analysis of differentially expressed transcripts in the absence of L1D1.

2025-01-01 | GSE254460 | GEO

RNA sequencing of control, DNMT1 knockout and DNMT1/L1TD1 double knockout haploid cells (HAP1)

Project description:To investigate the effects of L1TD1 ablation in DNMT1 knockout HAP-1 cells in comparison to DNMT1 knockout and control HAP1 cells

2024-12-25 | GSE169614 | GEO

RNA sequencing of control, DNMT1 knockout and DNMT1/L1TD1 double knockout haploid cells (HAP1)

Project description:RNA sequencing of control, DNMT1 knockout and DNMT1/L1TD1 double knockout haploid cells (HAP1)

| PRJNA717222 | ENA

Project description:L1TD1 RIP-seq analysis of DNMT1 KO and DNMT1 L1TD1 DKO HAP1 cells

| PRJNA1065915 | ENA

Dissolution of RNA condensates by the embryonic stem cell protein L1TD1 [CLIP-seq]

Project description:L1TD1 is a cytoplasmic RNA-binding protein that is specifically expressed in pluripotent stem cells and, unlike its mouse paralogue, is essential for the maintenance of stemness in human cells. Although L1TD1 is the only known domesticated gene from a LINE-1 (L1) retroelement, the functional legacy from its ancestral protein, ORF1p of L1, and the novelty are so far unknown. Here, we determined RNAs associated with L1TD1 and found that, like ORF1p, L1TD1 binds L1 RNAs, localizes to high-density ribonucleoprotein (RNP) condensates, and increases L1 retrotransposition. Unexpectedly, L1TD1 enhanced the translation of a subset of mRNAs enriched in the condensates. In HeLa cells, ectopically expressed L1TD1 facilitated the dissolution of stress granules and granules formed by pathological mutations of TDP-43 and FUS. The glutamate-rich domain and the ORF1-homology domain facilitated dispersal of the RNPs and induced autophagy, respectively. These results provide insights into how L1TD1 regulates gene expression in pluripotent stem cells. We propose that the ability of L1TD1 to dissolve stress granules may provide novel opportunities for treatments of neurodegenerative diseases caused by disturbed stress granule dynamics.

2023-04-26 | GSE227855 | GEO

Dissolution of RNA condensates by the embryonic stem cell protein L1TD1 [RNA-seq]

2023-04-26 | GSE227854 | GEO

Project description:Gene expression profile of DNMT1 KO and DNMT1 L1TD1 DKO HAP1 cell lines

| PRJNA1063551 | ENA

Gene expression changes in mouse forebrain deficient in Dnmt1 and Dnmt3a

Project description:Two major DNA methylation-catalyzing enzymes, Dnmt1 and Dnmt3a, are expressed in postmitotic neurons, but their function in the adult central nervous system is unclear. We generated conditional mutant mice (CamKIIa Cre; Dnmt loxP) that lack either Dnmt1 or Dnmt3a, or both, exclusively in forebrain excitatory neurons and found only double-knockout (DKO) mice exhibited abnormal hippocampal CA1 long-term plasticity and deficits of learning and memory. DKO neurons also exhibit a significant up-regulation of immune genes, such as class I MHC and Stat1, which are implicated in synaptic plasticity. Four pairs of 2-3 month-old DKO and litter mate control were used. RNA samples were extracted from the cortex and hippocampus for gene expression array analysis.

2010-12-03 | E-GEOD-19367 | biostudies-arrayexpress

Genome wide analysis of long term L1TD1 knockdown effects in NT2D1 cell line with inducible shRNA vectors

Project description:The purpose of this study was to analyze genome wide effects of long term L1TD1 silencing in embryonal carcinoma cell line (NT2D1) and to identify molecular level mechanismis of L1TD1 function and determine its possible role in pluripotency and self-renewal Six clones from NT2D1 cell line stably expressing TetR3 and L1TD1shRNA were analyzed. From each clone two samples were prepared: non-induced cells as control and doxycycline induced cells (+) to knockdown L1TD1, resulting altogether 12 samples. Samples were collected on day 6 after start of dox induction. One sample had to be excluded from the analysis because it did not cluster according to the sample groups.

2012-02-28 | E-GEOD-21275 | biostudies-arrayexpress

Gene expression changes in mouse forebrain deficient in Dnmt1 and Dnmt3a

2010-12-03 | GSE19367 | GEO