Transcriptomics

Dataset Information

0

Serine/arginine-rich splicing factor 7 (SRSF7) promotes the type I interferon response by activating Irf7 transcription


ABSTRACT: Tight regulation of macrophage immune gene expression is required to fight infection without risking harmful inflammation. The contribution of RNA binding proteins (RBPs) to shaping the macrophage response to pathogens remains poorly understood. Transcriptomic analysis revealed that a member of the serine/arginine-rich (SR) family of mRNA processing factors, SRSF7, is required for optimal expression of a cohort of interferon stimulated genes (ISGs) in macrophages. Using genetic and biochemical assays, we discovered that in addition to its canonical role in regulating alternative splicing, SRSF7 drives transcription of interferon regulatory transcription factor 7 (IRF7) to promote antiviral immunity. At the Irf7 promoter, SRSF7 maximizes STAT1 transcription factor binding and RNA polymerase II elongation via cooperation with the H4K20me1 histone methyltransferase KMT5a (SET8). These studies define an unorthodox role for an SR protein in activating transcription and reveal an unappreciated RNA binding protein-chromatin network that orchestrates macrophage antiviral gene expression.

ORGANISM(S): Mus musculus

PROVIDER: GSE254517 | GEO | 2024/02/05

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2024-07-29 | GSE239845 | GEO
2020-02-28 | PXD014257 | Pride
2012-09-19 | E-GEOD-37052 | biostudies-arrayexpress
2017-08-10 | GSE79792 | GEO
2012-09-19 | GSE36241 | GEO
2023-01-23 | GSE221996 | GEO
2021-09-09 | PXD018090 | Pride
2010-12-29 | E-GEOD-24708 | biostudies-arrayexpress
2012-09-19 | E-GEOD-36241 | biostudies-arrayexpress
2021-01-28 | GSE151722 | GEO