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Rare codon recoding for selective and efficient noncanonical amino acid incorporation [Ribo-seq]

ABSTRACT: The ability to genetically encode noncanonical amino acids (ncAAs) has empowered proteins with improved or novel properties. However, existing strategies in mammalian cells rely on the introduction of blank codon for incorporating ncAAs, which is very inefficient and limits their widespread applications. Here, we develop a rare codon recoding strategy that takes advantage of the relative rarity of the TCG codon to achieve highly selective and efficient ncAA incorporation through systematic engineering and big data model predictions. We highlight the broad utility of this strategy for the incorporation of dozens of ncAAs into various functional proteins at the wild-type protein expression levels, as well as the synthesis of proteins with up to 6-site ncAAs or 4 distinct ncAAs in mammalian cells for downstream applications.

ORGANISM(S): Homo sapiens

PROVIDER: GSE255465 | GEO | 2025/01/01

REPOSITORIES: GEO

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Rare codon recoding for selective and efficient noncanonical amino acid incorporation [RNA-seq]

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