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Asf1/HIRA facilitate global histone deacetylation and associate with HP1 to promote nucleosome occupancy at heterochromatic loci (expression)

ABSTRACT: Expression profile of Schizosaccharomyces pombe genome in asf1-1 and ∆hip1 cells. Expression profile of mutant cells compared to wild-type cells.

ORGANISM(S): Schizosaccharomyces pombe

PROVIDER: GSE25598 | GEO | 2011/01/07

SECONDARY ACCESSION(S): PRJNA142641

REPOSITORIES: GEO

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Asf1/HIRA facilitate global histone deacetylation and associate with HP1 to promote nucleosome occupancy at heterochromatic loci (expression)

Project description:Expression profile of Schizosaccharomyces pombe genome in asf1-1 and ∆hip1 cells. Expression profile of mutant cells compared to wild-type cells. Agilent 60mer oligonucleotide custom array containing probes spanning large portion of chromosome 2 at 50bp resolution was used to profile expression levels in mutant cells and to compare them to levels in wild-type cells.

2011-01-07 | E-GEOD-25598 | biostudies-arrayexpress

Asf1/HIRA facilitate global histone deacetylation and associate with HP1 to promote nucleosome occupancy at heterochromatic loci

Project description:This SuperSeries is composed of the following subset Series: GSE25597: Asf1/HIRA facilitate global histone deacetylation and associate with HP1 to promote nucleosome occupancy at heterochromatic loci (ChIP-chip) GSE25598: Asf1/HIRA facilitate global histone deacetylation and associate with HP1 to promote nucleosome occupancy at heterochromatic loci (expression) GSE25600: Asf1/HIRA facilitate global histone deacetylation and associate with HP1 to promote nucleosome occupancy at heterochromatic loci (MNase) Refer to individual Series

2011-01-07 | E-GEOD-25605 | biostudies-arrayexpress

Asf1/HIRA facilitate global histone deacetylation and associate with HP1 to promote nucleosome occupancy at heterochromatic loci.

Project description:Heterochromatin impacts various nuclear processes by providing a recruiting platform for diverse chromosomal proteins. In fission yeast, HP1 proteins Chp2 and Swi6, which bind to methylated histone H3 lysine 9, associate with SHREC (Snf2/HDAC repressor complex) and Clr6 histone deacetylases (HDACs) involved in heterochromatic silencing. However, heterochromatic silencing machinery is not fully defined. We describe a histone chaperone complex containing Asf1 and HIRA that spreads across silenced domains via its association with Swi6 to enforce transcriptional silencing. Asf1 functions in concert with a Clr6 HDAC complex to silence heterochromatic repeats, and it suppresses antisense transcription by promoting histone deacetylation. Furthermore, we demonstrate that Asf1 and SHREC facilitate nucleosome occupancy at heterochromatic regions but TFIIIC transcription factor binding sites within boundary elements are refractory to these factors. These analyses uncover a role for Asf1 in global histone deacetylation and suggest that HP1-associated histone chaperone promotes nucleosome occupancy to assemble repressive heterochromatin.

| S-EPMC3035992 | biostudies-literature

Asf1/HIRA facilitate global histone deacetylation and associate with HP1 to promote nucleosome occupancy at heterochromatic loci (MNase)

Project description:Nucleosome occupancy (NO) profiling in fission yeast Schizosaccharomyces pombe

2011-01-07 | GSE25600 | GEO

Asf1/HIRA facilitate global histone deacetylation and associate with HP1 to promote nucleosome occupancy at heterochromatic loci (ChIP-chip)

Project description:Occupancy profiling of H3K9ac in fission yeast Schizosaccharomyces pombe

2011-01-07 | GSE25597 | GEO

Asf1/HIRA facilitate global histone deacetylation and associate with HP1 to promote nucleosome occupancy at heterochromatic loci (MNase)

Project description:Nucleosome occupancy (NO) profiling in fission yeast Schizosaccharomyces pombe Agilent 60mer array was used to analyze mononucleosomal DNA recovered by MNase treatment from asynchronous culture of fission yeast.

2011-01-07 | E-GEOD-25600 | biostudies-arrayexpress

Asf1/HIRA facilitate global histone deacetylation and associate with HP1 to promote nucleosome occupancy at heterochromatic loci (ChIP-chip)

Project description:Occupancy profiling of H3K9ac in fission yeast Schizosaccharomyces pombe Agilent 60mer array was used to analyze DNA recovered by immunoprecipitation of H3k9ac from asynchronous culture of fission yeast.

2011-01-07 | E-GEOD-25597 | biostudies-arrayexpress

Asf1/HIRA facilitate global histone deacetylation and associate with HP1 to promote nucleosome occupancy at heterochromatic loci

Project description:This SuperSeries is composed of the SubSeries listed below.

2011-01-07 | GSE25605 | GEO

Cellular quiescence is dependent upon the HIRA histone chaperone

Project description:Quiescence (G0) is a reversible non-dividing state that facilitates cellular survival in adverse conditions. Here we demonstrate that the function of the HIRA histone chaperone complex is required for viability during nitrogen-starvation induced quiescence in Schizosaccharomyces pombe. G0 cells lacking the HIRA protein, Hip1 exhibit elevated levels of antisense ncRNAs and an increase in unrepaired DNA double strand breaks. Nitrogen-starved hip1∆ cells retain metabolic activity but, in contrast to wild type, rapidly lose the ability to resume proliferation. After a short period in G0 (1 day), hip1∆ mutants are able to resume cell growth in response to the restoration of a nitrogen source, but do not efficiently induce Start-specific gene expression and re-enter the cell cycle. However, hip1∆ cells rapidly progress to an unresponsive state and by 4 days in G0, the majority no longer initiate growth following nitrogen source restoration. Analysis using a conditional hip1 allele is consistent with these findings and indicates that HIRA is required for efficient exit from quiescence and prevents a permanent cell cycle arrest.

2023-11-13 | GSE129599 | GEO

Histone H4 acetylation in wild-type, ASF1, SET2 and ASF1 SET2 deletion yeast strains

Project description:ChIP-on chip assays to measure the change in histone acetylation over the yeast genome, in ASF1, SET2 and ASF1 SET2 deletion yeast strains compared to the wild-type control. ChIPs of AcH4 from wild-type, ASF1, SET2 and ASF1 SET2 deletion yeast strains were normalized to the H3 enrichment.

2012-07-25 | GSE28098 | GEO

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