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Synergistic activation by Glass and Pointed promotes neuronal identity in the Drosophila eye disc

ABSTRACT: The integration of extrinsic signaling with cell-intrinsic transcription factors can direct progenitor cells to differentiate into distinct cell fates.In the developingDrosophilaeye, differentiation of photoreceptors R1-R7 requires EGFR signaling mediated by the transcription factor Pointed, and our single-cell RNA-Seq analysis shows that the same photoreceptors require the eye-specific transcription factor Glass. We discovered that ectopic expression of Glass and activation of EGFR signaling synergistically induce neuronal gene expression in the wing disc in a Pointed-dependent manner. Targeted DamID reveals that Glass and Pointed share many binding sites in the genome of developing photoreceptors. Comparison with transcriptomic data shows that Pointed and Glass induce photoreceptor differentiation through intermediate transcription factors, including the redundant homologues Scratch and Scrape, as well as directly activating neuronal genes. Our data reveal synergistic activation of a multi-layered transcriptional network as the mechanism by which EGFR signaling induces neuronal identity in Glass-expressing cells.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE256221 | GEO | 2024/07/17

REPOSITORIES: GEO

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Synergistic activation by Glass and Pointed promotes neuronal identity in the Drosophila eye disc

Project description:Synergistic activation by Glass and Pointed promotes neuronal identity in the Drosophila eye disc

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