Genomics

Dataset Information

0

Neural tRNA Expression Patterns in Brain Organoids


ABSTRACT: While known for their canonical roles in protein translation, tRNAs and derived fragments have recently been ascribed additional functions in gene expression regulation. Despite recent evidence suggesting tRNA-Arg-TCT-4 plays an active role in mouse neural development, the extent to which it and other tRNAs function in early primate brain development remains unclear. Through the cultivation of cerebral cortical organoids and tRNA sequencing, we have identified groups of tRNAs and tRNA-derived small RNAs (tDRs) with dynamic expression patterns across early human cerebral cortex development. A wide diversity of tDRs were upregulated during cerebral cortex organoid generation, and derived from a wide range of isodecoders. Neuronally-active tDRs were clustered and classified based on abundance and fragment characteristics. From these groups, a few displayed dynamic expression throughout organoid neural differentiation. Furthermore, tDRs that increase over organoid development have sequence-specific conservation in common with Arg-TCT-4. This expanded collection of tDR transcripts and more comprehensive classification scheme should provide a framework for tRNA research in early neural development and help identify specific tDRs and key sequence features for targeted experimental study.

ORGANISM(S): Homo sapiens

PROVIDER: GSE259250 | GEO | 2025/01/13

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2020-10-01 | GSE149989 | GEO
2020-10-01 | GSE149970 | GEO
2020-10-01 | GSE149950 | GEO
2024-04-23 | PXD047423 | Pride
2024-07-31 | GSE255627 | GEO
2024-01-04 | GSE236623 | GEO
2024-01-04 | GSE236622 | GEO
2023-02-23 | GSE218457 | GEO
2023-12-12 | GSE249624 | GEO
2015-12-07 | E-GEOD-75140 | biostudies-arrayexpress