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CD45+/Col I+ Fibrocytes: Major Source of Collagen in the Fibrotic Lung, but not in Fibroblast Cultures

ABSTRACT: The role of cells of the hematopoietic lineage in fibrosis is controversial. Here we evaluate the contribution of Col I+/CD45+ cells (fibrocytes) to lung fibrosis. Systemic bleomycin treatment was used to induce fibrosis in a bone marrow transplant and two transgenic mouse models. Lung cells from these mice were analyzed by flow cytometry, both immediately upon release from the tissue or following growth on tissue-culture plastic. Fibrotic and control human lung tissue were also used. Fibroblasts and fibrocytes derived from a transgenic mouse model were compared in terms of their morphology, growth, and adhesion to fibronectin. Single cell RNAseq was performed with the analysis focusing on CD45-/Col I+ “fibroblasts” and CD45+/Col I+ “fibrocytes” in control and fibrotic mouse lung tissue. Finally, we inhibited fibrosis in mice using a novel, water-soluble version of caveolin scaffolding domain (CSD) called WCSD.

ORGANISM(S): Mus musculus

PROVIDER: GSE259367 | GEO | 2025/02/26

REPOSITORIES: GEO

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CD45+/Col I+ Fibrocytes: Major Source of Collagen in the Fibrotic Lung, but not in Fibroblast Cultures

Project description:CD45+/Col I+ Fibrocytes: Major Source of Collagen in the Fibrotic Lung, but not in Fibroblast Cultures

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