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Single-cell transcriptomic analysis of mouse lung cDC2s at various times post allergic sensitization

ABSTRACT: Conventionaldendritic cells (cDCs) in the lung that drive effector T cell differentiation, and initiate allergic responses upon inhalation of allergens. CD11b+type 2 cDCs (cDC2s) are heterogeneous, but the relationship among subpopulations has been elusive. To analyze their developmental pathways of cDC2 subpopulations, we performed single cell RNA-sequencing (scRNAS-Seq) of total lung cDC2s at various times following inhalation of house dust extracts, and detected multiple clusters based on their differential transcriptomes. The data of cDC2 transcriptome at steady state, early and later stages after activation will be applicable for the maturation pathway analysis of cDC2s.

ORGANISM(S): Mus musculus

PROVIDER: GSE261034 | GEO | 2024/06/05

REPOSITORIES: GEO

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Single-cell transcriptomic analysis of mouse lung CD11b+ dendritic cells post allergic sensitization

Project description:Conventional dendritic cells (cDCs) in the lung that drive effector T cell differentiation, and initiate allergic responses upon inhalation of allergens. However, since CD11b+ cDCs are heterogeneous, the specific function of each subpopulation has been elusive. To identify subpopulations of CD11b+ cDCs, we performed single cell RNA-sequencing (scRNAS-Seq) of total lung CD11b+ cDCs following inhalation of house dust extracts, and detected multiple clusters based on their differential transcriptomes. The data will be applicable for studies of cDC function in induction of T cell differentiation as well as the maturation pathway of cDCs.

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Single-cell transcriptomic analysis of mouse lung cDC2s at various times post allergic sensitization

Project description:Single-cell transcriptomic analysis of mouse lung cDC2s at various times post allergic sensitization

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