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Transcriptomic profiling of HBG gene expression in HEK293T cell line

ABSTRACT: Efficiency of HBG gene activation by the Cas12f/cgRNA system. In a doxycycline-inducible dCas12f-VPR knock-in (KI) HEK293T cell line, transiently transfected plasmids encoding U6, linear, and circular gRNAs targeting HBG1, and cgRNAs targeting mNeonGreen (negative control, NC) to activate HBG gene expression. RNA-seq analysis showed that cgRNAs exhibited increased efficiency in the target gene HBG1 and slight lower specificity when compared with U6 and linear gRNAs.

ORGANISM(S): Homo sapiens

PROVIDER: GSE261105 | GEO | 2024/03/12

REPOSITORIES: GEO

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