Transcriptomics

Dataset Information

0

Signal-independent activation reveals two-component regulatory networks.


ABSTRACT: Each bacterial species has specific regulatory systems to control physiology, adaptation, and host interactions. One challenge posed by this diversity is to define the evolving gene regulatory networks. This study aims to characterise two-component systems (TCS) in Streptococcus gagalactiae, the main cause of neonatal meningitis. Here we demonstrate signal-independent activation of signalling pathways by systematically targeting the conserved mechanism of phosphatase activity of the histidine kinases of the two main TCS families. Transcriptomic analysis resolves most pathways with high resolution, encompassing specialized, connected, and global regulatory systems. The activated network notably reveals the connection between CovRS and SaeRS signaling through the adhesin PbsP, linking the main regulators of host interactions to balance pathogenicity. Additionally, constitutive activation of the BceRS system reveals its role in cell envelope homeostasis beyond antimicrobial resistance. Overall, this study demonstrates the generalizability and versatility of TCS genetic activation to uncover regulatory logics and biological processes.

ORGANISM(S): Streptococcus agalactiae

PROVIDER: GSE261394 | GEO | 2024/03/25

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2012-03-09 | E-GEOD-36230 | biostudies-arrayexpress
2012-03-09 | GSE36230 | GEO
2022-08-11 | PXD028409 | Pride
2024-07-10 | GSE269249 | GEO
2021-10-01 | GSE184753 | GEO
2012-01-12 | E-GEOD-29309 | biostudies-arrayexpress
2018-02-06 | GSE109680 | GEO
| PRJNA908904 | ENA
2021-01-31 | GSE158512 | GEO
2012-01-12 | GSE29309 | GEO