Transcriptomics

Dataset Information

0

Circular RNA circRERE2 knockdown in rat primary cortical neurons [polyA RNA-seq]


ABSTRACT: Circular RNAs (circRNAs), a diverse class of ncRNAs highly enriched in developing neurons, play roles in local protein synthesis and synaptic plasticity. However, distinguishing functional from non-functional circRNAs is challenged by their abundance, tissue specificity and splicing variability. To address this, we conducted a RNAi knockdown screen targeting 32 highly expressed, conserved circRNAs enriched in dendritic processes. circRERE isoforms emerged as regulators of dendritic synapse density and electrophysiological characteristics. mRNA-seq supports the dysregulation of synaptic genes, particularly miR-128-3p-sensitive transcripts. MiR-128-3p activity and expression are reduced, with circRERE possessing multiple miR-128-3p binding sites, suggesting a protective interaction supported by a rescue of the synaptic phenotype upon miR-128-3p overexpression. Conversely, circRERE overexpression with intact miR-128-3p sites rescued the synaptic phenotype and independently increased miR-128-3p levels. These findings demonstrate the necessity for the broad characterization of circRNAs in the nervous system to comprehensively understand their influence on essential non-coding RNA regulatory networks.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE261607 | GEO | 2024/03/18

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2024-03-18 | GSE261609 | GEO
2020-09-10 | GSE157724 | GEO
2020-09-10 | GSE157723 | GEO
2017-03-29 | MSV000080762 | MassIVE
2018-02-23 | E-MTAB-5911 | biostudies-arrayexpress
2014-03-12 | E-GEOD-55777 | biostudies-arrayexpress
2014-03-12 | E-GEOD-55779 | biostudies-arrayexpress
2014-03-12 | E-GEOD-55780 | biostudies-arrayexpress
2014-03-12 | E-GEOD-55778 | biostudies-arrayexpress
2016-08-12 | PXD002172 | Pride