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Investigating the transcriptional heterogeneity of the beta cells experiencing Ca2+ excitotoxicity

ABSTRACT: Beta cells are remarkably heterogeneous. However, beta cells response to chronic stress at a single cell level remains largely unknown. Here, we used a chemo-genetic model of beta cell excitotoxcity coupled with single cell RNA sequencing to understand beta cells response to chronic stress. Remarkably, we found that a subset of stressed beta cells progressed towards death while the other group evaded the destruction by dedifferentiation. Vulnerable beta cells that progressed towards death showed upregulation of genes implicated in apoptosis, inflammation and immune system activation. The resilient beta cells that evaded the stress showed increased expression aldh1a3, a canonical marker of mammalian beta cell dedifferentiation.

ORGANISM(S): Danio rerio

PROVIDER: GSE261729 | GEO | 2024/10/24

REPOSITORIES: GEO

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Investigating the transcriptional heterogeneity of the beta cells experiencing Ca2+ excitotoxicity

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