Genomics

Dataset Information

0

Common and Specific Gene Regulatory Programs in Zebrafish Caudal Fin Regeneration at Single-Cell Resolution


ABSTRACT: Following amputation, zebrafish regenerate their injured caudal fin through lineage-restricted reprogramming. Although previous studies have charted various genetic and epigenetic dimensions of this process, the intricate gene regulatory programs shared by or unique to different regenerating cell types remain under-investigated. Here, we mapped the regulatory landscape of fin regeneration by applying paired snRNA-seq and snATAC-seq on uninjured and regenerating fin. This map delineates the regulatory dynamics of predominant cell populations at multiple stages of regeneration. We observed a marked increase in the accessibility of chromatin regions associated with regenerative and developmental processes at 1 dpa, followed by a gradual closure across major cell types at later stages. This pattern is distinct from that of transcriptomic dynamics which is characterized by several waves of gene upregulation and downregulation. We identified and in vivo validated cell type-specific and position-specific regeneration-responsive enhancers, and constructed regulatory networks by cell type and stage. Our single-cell resolution transcriptomic and chromatin accessibility map across regenerative stages provides new insights into regeneration regulatory mechanisms and serves as a valuable resource for the community.

ORGANISM(S): Danio rerio

PROVIDER: GSE261907 | GEO | 2024/11/01

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2024-05-01 | GSE261538 | GEO
2010-05-19 | E-GEOD-11391 | biostudies-arrayexpress
2021-07-17 | GSE158851 | GEO
2009-02-08 | E-GEOD-10766 | biostudies-arrayexpress
2016-06-01 | E-GEOD-74415 | biostudies-arrayexpress
2020-08-12 | GSE137971 | GEO
2023-10-13 | GSE242949 | GEO
2008-04-07 | E-GEOD-10184 | biostudies-arrayexpress
2008-04-07 | E-GEOD-10188 | biostudies-arrayexpress
2016-06-01 | GSE74415 | GEO