Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

LINE1 and PRC2 control nucleolar organization and repression of the 8C-state in human ESCs [RNA-seq_PXGL H9_L1KD]

ABSTRACT: In this study, we investigated LINE1 (L1), a major family of transposon elements (TEs) in the human genome, in naive hES cells for its roles in regulating 8-cell (8C) stage genes expression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE263287 | GEO | 2024/09/07

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

LINE1 and PRC2 control nucleolar organization and repression of the 8C-state in human ESCs [scRNA-seq]

Project description:In this study, we investigated LINE1 (L1), a major family of transposon elements (TEs) in the human genome, in naive hES cells for its roles in regulating 8-cell (8C) stage-specific totipotent genes. We performed single-cell RNA-seq (scRNA-seq) to assess if L1 knockdown in RSeT+DT naive hES cells induces 8-cell-like cells (8CLCs), and profile the transcriptome changes in the cells.

2024-09-07 | GSE232938 | GEO

LINE1 and PRC2 control nucleolar organization and repression of the 8C-state in human ESCs [RNA-Seq_RSeT+DT H9_EZH2i]

Project description:In this study, we investigated PRC2 (polycomb repressive complex 2), the H3K27me3 modifier, in naive hES cells for its roles in regulating 8-cell (8C) stage genes expression. EZH2 inhibitor, UNC1999 and its inactive analog UNC2400 were used for the treatment.

2024-09-07 | GSE263288 | GEO

LINE1 and PRC2 control nucleolar organization and repression of the 8C-state in human ESCs [H3K27me3 ChIP-seq,RSeT+DT_L1KD]

Project description:In this study, we performed H3K27me3 ChIP-seq to compare LINE1 knockdown naive hES cells with the control.

2024-09-09 | GSE263290 | GEO

LINE1 and PRC2 control nucleolar organization and repression of the 8C-state in human ESCs [ChIRP-seq,RSeT+DT H9]

Project description:In this study, we profiled for LINE1 binding loci in RSeT+DT naïve hES cells with the Chromatin Isolation by RNA Purification (ChIRP)-seq strategy. We followed a previously published ChIRP protocol described (Percharde et al. 2018; Lu et al. 2020).

2024-09-09 | GSE263289 | GEO

LINE1 and PRC2 control nucleolar organization and repression of the 8C-state in human ESCs [RNA-Seq_RSeT_H9_L1KD]

Project description:To study L1 knockdown effect in RSeT naïve hES cells

2024-09-07 | GSE232937 | GEO

LINE1 and PRC2 control nucleolar organization and repression of the 8C-state in human ESCs [RNA-Seq_Primed_H9_L1KD]

Project description:To study L1 knockdown effect in mTeSR primed hES cells

2024-09-07 | GSE232930 | GEO