Transcriptomics

Dataset Information

0

A Pvr–AP-1–Mmp1 signaling pathway is activated in astrocytes upon traumatic brain injury


ABSTRACT: Traumatic brain injury (TBI) caused by external mechanical forces is a major health burden worldwide, but the underlying mechanism in glia remains largely unclear. We report herein that Drosophila adults exhibit a defective blood-brain-barrier (BBB), elevated innate immune responses, and appear to be hypertrophy of astrocytes upon consecutive strikes with a high-impact trauma device. RNA sequencing (RNA-seq) analysis of these astrocytes revealed upregulated expression of genes encoding PDGF and VEGF receptor-related (Pvr, a receptor tyrosine kinase (RTK)), adaptor protein complex 1 (AP-1, a transcription factor complex of the c-Jun N-terminal Kinase (JNK) pathway) composed of Jun-related antigen (Jra) and kayak (kay), and matrix metalloproteinase 1 (Mmp1) following TBI. Interestingly, Pvr is both required and sufficient for AP-1 and Mmp1 upregulation, while knockdown of AP-1 expression in the background of Pvr overexpression in astrocytes rescued Mmp1 upregulation upon TBI, indicating that Pvr acts as the upstream receptor for the downstream AP-1–Mmp1 transduction. Moreover, dynamin-associated endocytosis was found to be an important regulatory step in downregulating Pvr signaling. Our results identify a new Pvr–AP-1–Mmp1 signaling pathway in astrocytes in response to TBI, providing potential targets for developing new therapeutic strategies of TBI.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE263447 | GEO | 2024/06/30

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

| PRJNA304162 | ENA
2024-10-21 | GSE279637 | GEO
2018-03-02 | GSE102367 | GEO
2015-05-10 | E-GEOD-55787 | biostudies-arrayexpress
2015-05-10 | GSE55787 | GEO
2021-06-01 | GSE167459 | GEO
2016-02-29 | E-GEOD-73372 | biostudies-arrayexpress
2009-02-20 | GSE10667 | GEO
2019-04-15 | GSE127564 | GEO
2018-03-02 | GSE102410 | GEO