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Gene expression profiling of mouse embryonic stem cells with single-cell RNA sequencing

ABSTRACT: Differences in gene-expression profiles between individual cells can give rise to distinct cell fate decisions. Yet how localisation on a micropattern impacts initial changes in mRNA, protein, and phosphoprotein abundance remains unclear. To identify the effect of cellular position on gene expression, we developed a scalable antibody and mRNA targeting sequential fluorescence in situ hybridization (ARTseq-FISH) method capable of simultaneously profiling mRNAs, proteins, and phosphoproteins in single cells. We studied 67 (phospho-)protein and mRNA targets in individual mouse embryonic stem cells (mESCs) cultured on circular micropatterns. To validate the detection of the mRNA targets quantified with ARTseq-FISH, we utilized single-cell RNA sequencing to profile a comparable population of mouse embryonic stem cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE264348 | GEO | 2024/04/24

REPOSITORIES: GEO

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