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Transcription profiling of One month old Gossypium herbaceum plant leaf during drought and watered condition

ABSTRACT: Comparative genomics has emerged as a promising means of unravelling the molecular networks underlying complex traits such as drought tolerance.We used Affymetrix microarray to detail the global programme of gene expression underlying in drought and watered condition and identified distinct classes of up-regulated genes during this process.leaf tissue were used for the expression analysis during watered and drought condition with three biological replicates for each case.

ORGANISM(S): Gossypium raimondii Gossypium barbadense Gossypium arboreum Gossypium herbaceum Gossypium hirsutum

PROVIDER: GSE26522 | GEO | 2011/01/11

SECONDARY ACCESSION(S): PRJNA136715

REPOSITORIES: GEO

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Transcription profiling of One month old Gossypium herbaceum plant leaf during drought and watered condition

Project description:Comparative genomics has emerged as a promising means of unravelling the molecular networks underlying complex traits such as drought tolerance.We used Affymetrix microarray to detail the global programme of gene expression underlying in drought and watered condition and identified distinct classes of up-regulated genes during this process.leaf tissue were used for the expression analysis during watered and drought condition with three biological replicates for each case. Here we assess the genotype-dependent component of the drought-induced transcriptome response in two contrasting cotton (Gossypium spp) genotypes differing in drought tolerance. Leaf tissue were used during drought and watered condtion for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain differential expression pattern during drought and wtered condition.

2011-01-11 | E-GEOD-26522 | biostudies-arrayexpress

Affymetrix Cotton Genome array expression data of root tissue of Gossypium herbaceum under drought stress and watered condition

2012-03-29 | GSE36249 | GEO

Affymetrix Cotton Genome array expression data of root tissue of Gossypium herbaceum under drought stress and watered condition

Project description:Genome-wide Transcriptional Analysis of Genes Associated with Drought Stress in Gossypium herbaceum root This experiment was designed to investigate the molecular mechanism associated with drought tolerance in root tissue of Gossypium herbaceum. The gene expression profiles of the root tissue using Affymetrix Cotton Genome Array were compared with drought tolerant and drought sensitive genotype of G.herbaceum under drought stress and watered condition. Many genes in various molecular function or biological processes were over- or under-represented between drought tolerant and sensitive genotype, suggesting various molecular mechanism and biochemical pathways are interlinked and tolerant genotype have developed multiple mechanisms as an adaptory behavior against drought stress. The transcriptional responses of root tissue in drought tolerant and sensitive genotype of Gossypium herbaceum under drought stress have been investigated. Physiological responses to drought stress, such as stomatal conductance, water use efficiency, root bending assay on different mannitiol concentration were also measured as indicators of imposed drought stress. Total RNA was isolated from root tissue from both genotype under drought stress and normal irrigated condition with three biological replicates

2012-03-28 | E-GEOD-36249 | biostudies-arrayexpress

Transcriptional profiling of Zea mays 10 DAP(days after pollination) kernels : water stress vs well-watered control

Project description:A customized targeted oligoarray was used to monitor the expression levels of 1000 genes, representative of the immature kernel transcriptome. Using this oligoarray we compared transcripts from 10 DAP kernels of two susceptible and two drought tolerant genotypes. These four genotypes were extracted from our RIL population (B73xH99) and grown under water stress and well watered field conditions. Keywords: Stress response Two-condition experiment: water stress field condition vs. well watered field condition. Transcriptional profiling of 10 DAP kernel (two susceptible and two drought tolerant genotypes) in the first condition vs 10 DAP kernel (two susceptible and two drought tolerant genotypes) in the second condition. Hybridization replicates: 4 in dye swap mode. Two probes per gene per array.

2008-12-21 | E-GEOD-11568 | biostudies-arrayexpress

Expression analysis in roots and leaves of asparagus bean accesions B47 and B128 under well-watered and soil drought conditions

Project description:Investigation of whole genome gene expression level changes in two asparagus bean accesions B47 and B128 under drought stress, compared to well-watered conditions. Two organs, leaf and root, were sampled for each accesion under both well-watered and drought conditions.

2014-11-26 | GSE63636 | GEO

Gossypium herbaceum

Project description:Transcription profiling of One month old Gossypium herbaceum plant leaf during drought and watered condition

| PRJNA136715 | ENA

Transcriptome analysis of Osbhlh148 mutant plants under controlled drought stress and well-watered conditions at vegetative stage

2015-01-16 | GSE65024 | GEO

Transcriptome analysis of Oshsfa2e mutant plants under controlled drought stress and well-watered conditions at vegetative stage

2015-01-16 | GSE65025 | GEO

Transcriptome analysis of Osbhlh148 mutant plants under controlled drought stress and well-watered conditions at vegetative stage

Project description:The members of bHLH transcription factor superfamily are known to play key role in plant development and abiotic stress response. Loss-of-function of OsbHLH148 gene resulted in increased sensitivity of rice plants to drought stress. To identify the targets of OsbHLH148 and dissect the drought stress response pathway regulated by it, we performed transcriptome profiling of Osbhlh148 mutant plants under drought stress as well as well-watered conditions by RNA-sequencing. OsbHLH148 loss-of-function rice plants (Oryza sativa ssp. japonica cv. Nipponbare) were exposed to controlled drought stress and well-watered conditions at the vegetative stage. Controlled drought (DR) stress was applied on 45 day old plants following gravimetric approach. The soil water content was brought down to 40% field capacity over a period of 3-4 days and plants were maintained at that level for 10 days by weighing the pots daily at a fixed time of the day and replenishing the water lost through evapotranspiration. Another set of plants were maintained at 100% FC as well-watered (WW) condition. Total RNA isolated from leaf tissue was used for RNA-sequencing. Two biological replicates per sample were sequenced. cDNA library was constructed using TruSeq Stranded Total RNA with Ribo-Zero Plant kit (Illumina). Sequencing was carried out on each library to generate 50 bp SE reads using Illumina High-Seq 2000 platform. The transcriptome reads were mapped to the rice reference genome sequence (MSU 7.0) with tophat1.3.1 using the program’s default parameters (http://tophat.cbcb.umd.edu). Mapped RNA-Seq reads were assembled into transcripts by Cufflinks (http://cufflinks.cbcb.umd.edu/) and differentially expressed genes were identified by using Cuffdiff.

2015-01-16 | E-GEOD-65024 | biostudies-arrayexpress

Transcriptome analysis of Oshsfa2e mutant plants under controlled drought stress and well-watered conditions at vegetative stage

Project description:Heat shock factors (Hsfs) are known to regulate heat and drought stress response by controlling the expression of heat shock proteins and oxidative stress responsive genes. Loss-of-function of OsHSFA2e gene resulted in increased sensitivity of rice plants to drought and heat stress. To identify the targets of OsHSFA2e and dissect the stress response pathway regulated by it, we performed transcriptome profiling of Oshsfa2e mutant plants under drought stress as well as well-watered conditions by RNA-sequencing. OsHSFA2e loss-of-function rice plants (Oryza sativa ssp. japonica cv. Nipponbare) were exposed to controlled drought stress and well-watered conditions at the vegetative stage. Controlled drought (DR) stress was applied on 45 day old plants following gravimetric approach. The soil water content was brought down to 40% field capacity over a period of 3-4 days and plants were maintained at that level for 10 days by weighing the pots daily at a fixed time of the day and replenishing the water lost through evapotranspiration. Another set of plants were maintained at 100% FC as well-watered (WW) condition. Total RNA isolated from leaf tissue was used for RNA-sequencing. Two biological replicates per sample were sequenced. cDNA library was constructed using TruSeq Stranded Total RNA with Ribo-Zero Plant kit (Illumina). Sequencing was carried out on each library to generate 50 bp SE reads using Illumina High-Seq 2000 platform. The transcriptome reads were mapped to the rice reference genome sequence (MSU 7.0) with tophat1.3.1 using the program’s default parameters (http://tophat.cbcb.umd.edu). Mapped RNA-Seq reads were assembled into transcripts by Cufflinks (http://cufflinks.cbcb.umd.edu/) and differentially expressed genes were identified by using Cuffdiff.

2015-01-16 | E-GEOD-65025 | biostudies-arrayexpress

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