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Chloroplast precursor protein overaccumulation triggers multilevel reprogramming and a heat shock-like response in plant cells [Ribo-Seq]

ABSTRACT: Most of chloroplast proteins are encoded by nucleus ,synthesied in cytosol as precursors. theses precursor contains tranzit peptide and will be cut off after import finished. How plant response to chloroplast precursor overaccumulation stress(cPOS) is still unkown. Here we constructed constutitive and induced overexpression line to lead preClpD accumulation. Proteomic analysis revealed cytosolic ribosome upregulated, which means translation changed upon cPOS. So we conducted RNA-seq and Ribo-seq experiment to find which genes response at transcription level or translation level.Finally, we want to uncover the cellular events upon chloroplast precursor overaccumulation.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE265859 | GEO | 2025/03/19

REPOSITORIES: GEO

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Chloroplast precursor protein overaccumulation triggers multilevel reprogramming and a heat shock-like response in plant cells [RNA-Seq]

Project description:Most of chloroplast proteins are encoded by nucleus ,synthesied in cytosol as precursors. theses precursor contains tranzit peptide and will be cut off after import finished. How plant response to chloroplast precursor overaccumulation stress(cPOS) is still unkown. Here we constructed constutitive and induced overexpression line to lead preClpD accumulation. Proteomic analysis revealed cytosolic ribosome upregulated, which means translation changed upon cPOS. So we conducted RNA-seq and Ribo-seq experiment to find which genes response at transcription level or translation level.Finally, we want to uncover the cellular events upon chloroplast precursor overaccumulation.

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Chloroplast precursor protein overaccumulation triggers multilevel reprogramming and a heat shock-like response in plant cells

Project description:Most of chloroplast proteins are encoded by nucleus ,synthesied in cytosol as precursors. theses precursor contains tranzit peptide and will be cut off after import finished. How plant response to chloroplast precursor overaccumulation stress(cPOS) is still unkown. Here we constructed constutitive and induced overexpression line to lead preClpD accumulation. Proteomic analysis revealed cytosolic ribosome increase, which means translation changed upon cPOS. So we conducted RNA-seq and Ribo-seq experiment to find which genes response at transcription level or translation level.Finally, we want to uncover the cellular events upon chloroplast precursor overaccumulation.

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Chloroplast precursor protein overaccumulation triggers multilevel reprogramming and a heat shock-like response in plant cells [Ribo-Seq]

Project description:Chloroplast precursor protein overaccumulation triggers multilevel reprogramming and a heat shock-like response in plant cells [Ribo-Seq]

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