Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptional profile of Salmonella enterica serovars Kentucky and Enteritidis in response to acidic conditions

ABSTRACT: S. enterica sv Kentucky 3795 and S. enterica sv Enteritidis NalR were grown to mid-log phase in TSB, then subjected to three different acidic conditions generated by two different acids: HCl to pH4.5, HCl to pH5.5 and CH3COOH to pH5.5. After 10min, total RNA was harvested und compared to total RNA harvested from identical control cultures grown in TSB without the pH alteration.

ORGANISM(S): Salmonella enterica Salmonella enterica subsp. enterica serovar Kentucky Salmonella enterica subsp. enterica serovar Enteritidis

PROVIDER: GSE26597 | GEO | 2011/02/01

SECONDARY ACCESSION(S): PRJNA136197

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Shared Molecules

Only show the datasets with similarity scores above: 0.5

Threshold

0.5

Similar Datasets

Transcriptional profile of Salmonella enterica serovars Kentucky and Enteritidis in response to acidic conditions

Project description:S. enterica sv Kentucky 3795 and S. enterica sv Enteritidis NalR were grown to mid-log phase in TSB, then subjected to three different acidic conditions generated by two different acids: HCl to pH4.5, HCl to pH5.5 and CH3COOH to pH5.5. After 10min, total RNA was harvested und compared to total RNA harvested from identical control cultures grown in TSB without the pH alteration. At least three biological replicates were performed for each strain and acidic condition. Total RNAs were harvested, and labeled by the conventional protocol of Brown et al, then hybridized onto a Salmonella-specific PCR product array that covered over 97% of the Enteritidis genome.

2011-02-01 | E-GEOD-26597 | biostudies-arrayexpress

S.enterica_log_vs_overnight_DNA

Project description:Gene content comparison of Salmonella enterica sv Typhimurium LT2, sv Typhi CT18 and sv Typhi TY2 cultures grown to log phase versus cultures grown overnight Keywords: parallel sample

2004-05-10 | GSE819 | GEO

Salmonella enterica LT2 microbeMASST submission

Project description:HLB SPE extract from S. enterica LT2 grown in TSB at 37C for 24hr. Media blank is included in supplementary files.

2022-07-31 | MSV000090032 | MassIVE

S.enterica_log_vs_overnight_DNA

Project description:Gene content comparison of Salmonella enterica sv Typhimurium LT2, sv Typhi CT18 and sv Typhi TY2 cultures grown to log phase versus cultures grown overnight

2010-06-09 | E-GEOD-819 | biostudies-arrayexpress

Characterisation of Modular Reponse of non-pathogenic E.coli O157 Sakai Response to acid adaptation

Project description:To study the dynamics of acid adaptation in E.coli at pH5.5 as opposed to that at pH7, samples were collected from a steady-state system for transcriptomics. Keywords: time course Single channel hybridisation were carried out using cy5 dye. Samples were collected from steady state system at pH7 and pH5.5 for 2.5h at 0min, 10min, 30min, 1h, 1.5h, 2h, 2.5h

2014-09-01 | E-GEOD-13180 | biostudies-arrayexpress

Characterisation of Modular Reponse of non-pathogenic E.coli K12 MG1655 Response to acid adaptation, part B

2014-09-01 | E-GEOD-13179 | biostudies-arrayexpress

Salmonella enterica serovar Typhimurium SL1344 cells: wildtype vs. ΔfabR mutant

Project description:Transcriptional profiling of Salmonella enterica serovar Typhimurium SL1344 cells comparing wildtype with ΔfabR mutant. Salmonella strains were cultured under free-living TSB conditions (TSB 1/20, 200 rpm, 25 °C) for 6h (ca. 2 x 108 cells/ml).

2014-12-31 | GSE52880 | GEO

Identification of Salmonella genes regulated by STM2239

Project description:Transcriptional profile of Salmonella enterica sv Typhimurium SL1344 M-NM-^TSTM2239 mutant cultures containing either a plasmid control (pFLAG-CTC) or a plasmid carrying STM2239 (pFLAG-STM2239) in the presence of IPTG. Total RNA was harvested from three biological replicates of M-NM-^TSTM2239 mutant cultures with either a plasmid control (pFLAG-CTC) or a plasmid carrying STM2239 (pFLAG-STM2239) that were grown in LB to OD 0.5 with 1mM IPTG to induce plasmid-driven expression.

2013-05-23 | E-GEOD-47165 | biostudies-arrayexpress

The nsrR regulon in Salmonella Typhimurium 14028s

Project description:In this study, we have defined the NsrR regulon in Salmonella enterica sv. Typhimurium 14028s using a transcriptional microarray. Wild-type and nsrR mutant S. Typhimurium were grown aerobically to early log-phase (OD600~0.5) at 37C in LB medium. Total RNA was isolated from three independent cultures of both strains and interrogated on a PCR product array representing almost all ORFs.

2012-11-21 | GSE32585 | GEO

SL1344_heat_shock_42C_RNA

Project description:Gene expression in Salmonella enterica sv Typhimurium SL1344 after heat shock to 42C Keywords: time-course

2004-05-10 | GSE808 | GEO